Nuclear scaffold attachment sites in the human globin gene complexes

In an analysis of a 90‐kb region around the human beta‐globin gene complex we have identified at least eight sites of attachment to the nuclear scaffold (SARs). While these have many potential functions, there appears to be a particular association with sequences important in the regulation of the c...

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Bibliographic Details
Published in:The EMBO journal 1988-11, Vol.7 (11), p.3337-3344
Main Authors: Jarman, A. P., Higgs, D. R.
Format: Article
Language:English
Subjects:
Binding Sites
Biological and medical sciences
Blotting, Southern
Cell Nucleus - metabolism
DNA - analysis
DNA - genetics
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation
Globins - genetics
Humans
Molecular and cellular biology
Molecular genetics
Multigene Family
Nucleic Acid Hybridization
Restriction Mapping
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Summary:In an analysis of a 90‐kb region around the human beta‐globin gene complex we have identified at least eight sites of attachment to the nuclear scaffold (SARs). While these have many potential functions, there appears to be a particular association with sequences important in the regulation of the complex. Two SARs are close to the known enhancer‐like elements of the beta‐globin gene. SARs flanking the complex co‐habit with the boundaries of the putative beta‐like globin gene regulatory domain. In contrast, we have detected no SARs within a 140‐kb region of the human alpha‐globin gene complex. If SARs play a role in the regulation of gene expression then this structural difference would imply a difference in the regulation of the two complexes.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1988.tb03205.x