Fibrinogen matrix deposited on the surface of biomaterials acts as a natural anti-adhesive coating

• Published in: Biomaterials 2015-10, Vol.67, p.151-159
• Main Authors: Safiullin, Roman, Christenson, Wayne, Owaynat, Hadil, Yermolenko, Ivan S, Kadirov, Marsil K, Ros, Robert, Ugarova, Tatiana P
• Format: Article
• Language: English
• Subjects: Adhesion; Adhesives - pharmacology; Adsorption; Advanced Basic Science; Biomaterials; Biomedical materials; Cell Adhesion - drug effects; Coated Materials, Biocompatible - pharmacology; Dentistry; Extracellular Matrix - metabolism; Fibrinogen; Fibrinogen - pharmacology; Grafting; HEK293 Cells; Hemocompatibility; Humans; Microscopy, Electron, Scanning; Platelet adhesion; Platelet Adhesiveness - drug effects; Polyethylene Terephthalates - pharmacology; Polytetrafluoroethylene - pharmacology; Spectrum Analysis; Surface chemistry; Surface Properties; Surgical implants; Thrombogenicity; U937 Cells; Vascular graft
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2015.07.007

Abstract Adsorption of fibrinogen on the luminal surface of biomaterials is a critical early event during the interaction of blood with implanted vascular graft prostheses which determines their thrombogenicity. We have recently identified a nanoscale process by which fibrinogen modifies the adhesive properties of various surfaces for platelets and leukocytes. In particular, adsorption of fibrinogen at low density promotes cell adhesion while its adsorption at high density results in the formation of an extensible multilayer matrix, which dramatically reduces cell adhesion. It remains unknown whether deposition of fibrinogen on the surface of vascular graft materials produces this anti-adhesive effect. Using atomic force spectroscopy, single cell force spectroscopy, and standard adhesion assays with platelets and leukocytes, we have characterized the adhesive and physical properties of the contemporary biomaterials, before and after coating with fibrinogen. We found that uncoated PET, PTFE and ePTFE exhibited high adhesion forces developed between the AFM tip or cells and the surfaces. Adsorption of fibrinogen at the increasing concentrations progressively reduced adhesion forces, and at ≥2 μg/ml all surfaces were virtually nonadhesive. Standard adhesion assays performed with platelets and leukocytes confirmed this dependence. These results provide a better understanding of the molecular events underlying thrombogenicity of vascular grafts.