Proteomic Analysis of Saliva from Patients with Oral Chronic Graft-Versus-Host Disease

• Published in: Biology of blood and marrow transplantation 2014-07, Vol.20 (7), p.1048-1055
• Main Authors: Devic, Ivana, Shi, Min, Schubert, Mark M, Lloid, Michele, Izutsu, Kenneth T, Pan, Catherine, Missaghi, Melody, Morton, Thomas H, Mancl, Lloyd A, Zhang, Jing, Presland, Richard B
• Format: Article
• Language: English
• Subjects: Adult; Aged; Biomarker discovery; Chronic Disease; Chronic graft-versus-host disease; Female; Graft vs Host Disease - metabolism; Hematology, Oncology and Palliative Medicine; Humans; iTRAQ mass spectrometry; Male; Mass Spectrometry; Middle Aged; Mouth Diseases - metabolism; Mouth Mucosa - metabolism; Proteomics; Proteomics - methods; Saliva; Saliva - chemistry; Saliva - metabolism
• ISSN: 1083-8791; 1523-6536
• DOI: 10.1016/j.bbmt.2014.03.031

Abstract Chronic graft-versus-host disease (cGVHD) is an immune-mediated disorder and is the major long-term complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT). The oral mucosa, including the salivary glands, is affected in the majority of patients with cGVHD; however, at present there is only a limited understanding of disease pathobiology. In this study, we performed a quantitative proteomic analysis of saliva pooled from patients with and without oral cGVHD—cGVHD(+) and cGVHD(−), respectively—using isobaric tags for relative and absolute quantification labeling, followed by tandem mass spectrometry. Among 249 salivary proteins identified by tandem mass spectrometry, 82 exhibited altered expression in the oral cGVHD(+) group compared with the cGVHD(−) group. Many of the identified proteins function in innate or acquired immunity, or are associated with tissue maintenance functions, such as proteolysis or the cytoskeleton. Using ELISA immunoassays, we further confirmed that 2 of these proteins, IL-1 receptor antagonist and cystatin B, showed decreased expression in patients with active oral cGVHD ( P