Transcription of Histone H4, H3, and H1 Cell Cycle Genes: Promoter Factor HiNF-D Contains CDC2, Cyclin A, and an RB-Related Protein

• Published in: Proceedings of the National Academy of Sciences - PNAS 1994-12, Vol.91 (26), p.12882-12886
• Main Authors: van Wijnen, Andre J., Aziz, Farah, Grana, Xavier, De Luca, Antonio, Desai, Rajesh K., Jaarsveld, Karen, Last, Thomas J., Soprano, Kenneth, Giordano, Antonio, Lian, Jane B., Stein, Janet L., Stein, Gary S.
• Format: Article
• Language: English
• Subjects: Alkaloids - pharmacology; Antibodies; Antiserum; Base Sequence; Biochemistry; Cdc2 kinase; CDC2 Protein Kinase - metabolism; Cell Cycle; Cellular biology; cyclin A; Cyclins; Cyclins - metabolism; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA-Binding Proteins - chemistry; DNA-Binding Proteins - metabolism; Ethers, Cyclic - pharmacology; Gels; Gene Expression Regulation; Genes; HeLa cells; HiNF-D protein; histone H1; histone H3; Histones; Histones - genetics; Humans; In Vitro Techniques; Macromolecular Substances; man; Medical research; Molecular Sequence Data; Okadaic Acid; Oligodeoxyribonucleotides - chemistry; Oligonucleotides; Phosphorylation; Promoter Regions, Genetic; Rb-related protein; Retinoblastoma Protein - chemistry; RNA, Messenger - genetics; Staurosporine; Transcription Factors - chemistry; Transcription, Genetic; Transcriptional Activation
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.91.26.12882

Cell cycle-controlled human histone genes are coordinately expressed during S phase, and transcriptional regulation involves a series of trans-acting factors (HiNFs). The proliferation-specific factor HiNF-D interacts with multiple recognition motifs in histone H4, H3, and H1 promoters. Using gel shift immunoassays, we show that CDC2, cyclin A, and an RB-related protein are ubiquitous subunits of HiNF-D binding activity isolated from several cell types. HiNF-D levels in vivo are sensitive to okadaic acid and staurosporine, indicating that HiNF-D activity and/or assembly is influenced by phosphorylation status. Thus, HiNF-D appears to be a multicomponent phosphoprotein that participates in coordinate control of multiple histone H4, H3, and H1 genes during the cell cycle. The presence of cell cycle mediators in the HiNF-D complex suggests linkage between transcriptional control of histones, enzymes involved in DNA synthesis, and the onset of DNA replication during the G1/S phase transition.