Evaluation of dual-wavelength excitation autofluorescence imaging of colorectal tumours with a high-sensitivity CMOS imager: a cross-sectional study

• Published in: BMC gastroenterology 2015-09, Vol.15 (1), p.110-110, Article 110
• Main Authors: Kominami, Yoko, Yoshida, Shigeto, Tanaka, Shinji, Miyaki, Rie, Sanomura, Yoji, Seo, Min-Woong, Kagawa, Keiichiro, Kawahito, Shoji, Arimoto, Hidenobu, Yamada, Kenji, Chayama, Kazuaki
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - diagnosis; Adenoma - diagnosis; Aged; Colonic Polyps - diagnosis; Colorectal Neoplasms - diagnosis; Cross-Sectional Studies; Female; Fluorescence; Humans; Male; Middle Aged; Optical Imaging - instrumentation; Optical Imaging - methods; Semiconductors; Technical Advance
• ISSN: 1471-230X; 1471-230X
• DOI: 10.1186/s12876-015-0339-6

It is important to devise efficient and easy methods of detecting colorectal tumours to reduce mortality from colorectal cancer. Dual-wavelength excitation autofluorescence intensity can be used to visualize colorectal tumours. Therefore, we evaluated dual-wavelength excitation autofluorescence images of colorectal tumours obtained with a newly developed, high-sensitivity complementary metal-oxide-semiconductor (CMOS) imager. A total 107 colorectal tumours (44 adenomas, 43 adenocarcinomas with intramucosal invasion, and 20 sessile serrated adenoma/polyps [SSA/Ps]) in 98 patients who underwent endoscopic tumour resection were included. The specimens were irradiated with excitation light at 365 nm and 405 nm, and autofluorescence images measured with a 475 ± 25-nm band pass filter were obtained using a new, high-sensitivity CMOS imager. Ratio images (F365ex/F405ex) were created to evaluate the lesion brightness compared with that of normal mucosa, and specimens were categorized into a no signal or high signal group. Adenomas and adenocarcinomas were depicted in 87 ratio images, with 86.2% (n = 75) in the High signal group. SSA/P was depicted in 20 ratio images, with 70.0% (n = 14) in the High signal group. Dual-wavelength excitation autofluorescence images of colorectal tumours can be acquired using our high-sensitivity CMOS imager, and are useful in detecting colorectal tumours.