Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma

Here, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated. The Apoptosis was evidenced by investigating DNA fragmentation, and Acri...

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Bibliographic Details
Published in:BMC complementary and alternative medicine 2015-09, Vol.15 (1), p.300-300, Article 300
Main Authors: Boubaker, Jihed, Mokdad Bzeouich, Imen, Nasr, Nouha, Ben Ghozlen, Hajer, Mustapha, Nadia, Ghedira, Kamel, Chekir-Ghedira, Leila
Format: Article
Language:English
Subjects:
acridine orange
Animals
Antineoplastic Agents - pharmacology
Apoptosis
bioactive properties
Bioflavonoids
Care and treatment
Cell Line, Tumor
Cell Proliferation - drug effects
chemical constituents of plants
complement
DNA fragmentation
ethidium
Flavones
Flavonoids
Free radicals (Chemistry)
leaves
Magnoliopsida - chemistry
melanin
melanogenesis
Melanoma
Melanoma, Experimental - metabolism
Mice
Nitraria retusa
phytochemicals
Plant Extracts - pharmacology
Plant Leaves - chemistry
Polyphenols
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Summary:Here, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated. The Apoptosis was evidenced by investigating DNA fragmentation, and Acridine orange/ethidium bromide staining. Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm. Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation. Our result was confirmed by ladder DNA fragmentation profile. All extracts showed also the ability to enhance melanogenesis and tyrosinase activity of B16F10 melanoma cells. The tested extracts have a significant biological effect which may be due to their bioactive compounds.
ISSN:1472-6882
1472-6882
DOI:10.1186/s12906-015-0743-z