Transglutaminase 2—a novel inhibitor of adipogenesis

• Published in: Cell death & disease 2015-08, Vol.6 (8), p.e1868-e1868
• Main Authors: Myneni, V D, Melino, G, Kaartinen, M T
• Format: Article
• Language: English
• Subjects: 13/1; 38/39; 631/45/607; 631/80/86; 692/699/2743/393; 82/51; 96/100; 96/106; Actins - genetics; Actins - metabolism; Adipocytes - cytology; Adipocytes - metabolism; Adipogenesis - genetics; Adipose Tissue, White - cytology; Adipose Tissue, White - metabolism; Animals; Antibodies; beta Catenin - genetics; beta Catenin - metabolism; Biochemistry; Biomedical and Life Sciences; Calcium-Binding Proteins; CCAAT-Enhancer-Binding Proteins - genetics; CCAAT-Enhancer-Binding Proteins - metabolism; Cell Biology; Cell Culture; Cell Differentiation; Embryo, Mammalian; Fibroblasts - cytology; Fibroblasts - metabolism; Gene Expression Regulation; GTP-Binding Proteins - deficiency; GTP-Binding Proteins - genetics; Immunology; Intercellular Signaling Peptides and Proteins - genetics; Intercellular Signaling Peptides and Proteins - metabolism; Life Sciences; Mice; Mice, Knockout; Original; original-article; Phosphorylation; PPAR gamma - genetics; PPAR gamma - metabolism; Protein Glutamine gamma Glutamyltransferase 2; Protein Transport; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; rho-Associated Kinases - genetics; rho-Associated Kinases - metabolism; Signal Transduction; Transglutaminases - deficiency; Transglutaminases - genetics
• ISSN: 2041-4889; 2041-4889
• DOI: 10.1038/cddis.2015.238

Differentiation of preadipocytes to lipid storing adipocytes involves extracellular signaling pathways, matrix remodeling and cytoskeletal changes. A number of factors have been implicated in maintaining the preadipocyte state and preventing their differentiation to adipocytes. We have previously reported that a multifunctional and protein crosslinking enzyme, transglutaminase 2 (TG2) is present in white adipose tissue. In this study, we have investigated TG2 function during adipocyte differentiation. We show that TG2 deficient mouse embryonic fibroblasts ( Tgm2 −/− MEFs) display increased and accelerated lipid accumulation due to increased expression of major adipogenic transcription factors, PPARγ and C/EBPα . Examination of Pref-1/Dlk1, an early negative regulator of adipogenesis, showed that the Pref-1/Dlk1 protein was completely absent in Tgm2 −/− MEFs during early differentiation. Similarly, Tgm2 −/− MEFs displayed defective canonical Wnt/β-catenin signaling with reduced β-catenin nuclear translocation. TG2 deficiency also resulted in reduced ROCK kinase activity, actin stress fiber formation and increased Akt phosphorylation in MEFs, but did not alter fibronectin matrix levels or solubility. TG2 protein levels were unaltered during adipogenic differentiation, and was found predominantly in the extracellular compartment of MEFs and mouse WAT. Addition of exogenous TG2 to Tgm2 +/+ and Tgm2 −/− MEFs significantly inhibited lipid accumulation, reduced expression of PPARγ and C/EBPα , promoted the nuclear accumulation of β-catenin, and recovered Pref-1/Dlk1 protein levels. Our study identifies TG2 as a novel negative regulator of adipogenesis.