Implementation and application of a multiplex assay to detect malaria-specific antibodies: a promising tool for assessing malaria transmission in Southeast Asian pre-elimination areas

Epidemiological surveillance is a key activity in malaria control and elimination in low-transmission and pre-elimination settings. Hence, sensitive tools for estimating malaria force of infection are crucial. Serological markers might provide additional information in estimating force of infection...

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Bibliographic Details
Published in:Malaria journal 2015-09, Vol.14 (1), p.338-338, Article 338
Main Authors: Kerkhof, Karen, Canier, Lydie, Kim, Saorin, Heng, Somony, Sochantha, Tho, Sovannaroth, Siv, Vigan-Womas, Inès, Coosemans, Marc, Sluydts, Vincent, Ménard, Didier, Durnez, Lies
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Analysis
Anopheles
Antibodies, Protozoan - blood
Antigens
Biochemistry, Molecular Biology
Cambodia - epidemiology
Cellular Biology
Child
Child, Preschool
Health aspects
Human health and pathology
Humans
Immunoassay - methods
Immunology
Infant
Infectious diseases
Life Sciences
Malaria, Falciparum - epidemiology
Malaria, Falciparum - immunology
Malaria, Falciparum - transmission
Malaria, Vivax - epidemiology
Malaria, Vivax - immunology
Malaria, Vivax - transmission
Medical research
Medicine, Experimental
Middle Aged
Parasitology - methods
Plasmodium falciparum - immunology
Plasmodium vivax - immunology
Recombinant proteins
Reproducibility of Results
Santé publique et épidémiologie
Young Adult
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Summary:Epidemiological surveillance is a key activity in malaria control and elimination in low-transmission and pre-elimination settings. Hence, sensitive tools for estimating malaria force of infection are crucial. Serological markers might provide additional information in estimating force of infection in low-endemic areas along with classical parasite detection methods. Serological markers can be used to estimate recent, past or present malaria exposure, depending on the used markers and their half-life. An assay based on 14 Plasmodium-specific peptides, one peptide specific for Anopheles gambiae saliva protein and five Plasmodium-specific recombinant proteins was developed for the MAGPIX system, assessed for its performance, and applied on blood spots from 2000 individuals collected in the Ratanakiri Province, Cambodia. A significant correlation for the use of 1000 and 2000 beads/antigen/well as well as for the monoplex versus multiplex assay was observed for all antigens (p 
ISSN:1475-2875
1475-2875
DOI:10.1186/s12936-015-0868-z