Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome

The serial coupling of a reversed-phase liquid chromatography (RPLC) column to a hydrophilic interaction liquid chromatography (HILIC) column has been developed in recent years for the detection of polar and nonpolar metabolites. TCA intermediates, bile acid standards and numerous polar and non-pola...

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Bibliographic Details
Published in:Metabolomics 2015, Vol.11 (5), p.1465-1470
Main Authors: Haggarty, Jennifer, Oppermann, Madalina, Dalby, Matthew J., Burchmore, Richard J., Cook, Ken, Weidt, Stefan, Burgess, Karl E. V.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
Developmental Biology
Life Sciences
Molecular Medicine
Short Communication
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Summary:The serial coupling of a reversed-phase liquid chromatography (RPLC) column to a hydrophilic interaction liquid chromatography (HILIC) column has been developed in recent years for the detection of polar and nonpolar metabolites. TCA intermediates, bile acid standards and numerous polar and non-polar metabolites extracted from beer were analysed using a combined RPLC/HILIC method. Non-polar metabolites were retained by the RPLC column. Polar metabolites not retained by the RPLC column were retained and separated by the HILIC column. The results from this study validate this simple yet powerful metabolomics approach.
ISSN:1573-3882
1573-3890
DOI:10.1007/s11306-014-0770-7