Mycofabricated biosilver nanoparticles interrupt Pseudomonas aeruginosa quorum sensing systems

Quorum sensing (QS) is a chemical communication process that Pseudomonas aeruginosa uses to regulate virulence and biofilm formation. Disabling of QS is an emerging approach for combating its pathogenicity. Silver nanoparticles (AgNPs) have been widely applied as antimicrobial agents against human p...

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Bibliographic Details
Published in:Scientific reports 2015-09, Vol.5 (1), p.13719-13719, Article 13719
Main Authors: Singh, Braj R., Singh, Brahma N., Singh, Akanksha, Khan, Wasi, Naqvi, Alim H., Singh, Harikesh B.
Format: Article
Language:English
Subjects:
631/326
692/700
Alginic acid
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - chemistry
Antimicrobial agents
Biofilms
Biofilms - drug effects
Chemical communication
Fungi
Fungi - chemistry
Humanities and Social Sciences
Metabolites
Metal Nanoparticles - administration & dosage
Metal Nanoparticles - chemistry
Models, Biological
multidisciplinary
Nanoparticles
Pathogenicity
Pathogens
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - physiology
Pyocyanin
Quorum Sensing - drug effects
Science
Secretion
Silver
Silver - chemistry
Tobramycin - pharmacology
Transcription
Virulence factors
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Summary:Quorum sensing (QS) is a chemical communication process that Pseudomonas aeruginosa uses to regulate virulence and biofilm formation. Disabling of QS is an emerging approach for combating its pathogenicity. Silver nanoparticles (AgNPs) have been widely applied as antimicrobial agents against human pathogenic bacteria and fungi, but not for the attenuation of bacterial QS. Here we mycofabricated AgNPs (mfAgNPs) using metabolites of soil fungus Rhizopus arrhizus BRS-07 and tested their effect on QS-regulated virulence and biofilm formation of P . aeruginosa . Transcriptional studies demonstrated that mfAgNPs reduced the levels of LasIR-RhlIR. Treatment of mfAgNPs inhibited biofilm formation, production of several virulence factors (e.g. LasA protease, LasB elastrase, pyocyanin, pyoverdin, pyochelin, rhamnolipid and alginate) and reduced AHLs production. Further genes quantification analyses revealed that mfAgNPs significantly down-regulated QS-regulated genes, specifically those encoded to the secretion of virulence factors. The results clearly indicated the anti-virulence property of mfAgNPs by inhibiting P . aeruginosa QS signaling.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep13719