Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serum

Bone marrow-derived mesenchymal stromal cells (MSCs) have been intensely studied for the purpose of developing solutions for clinical tissue engineering. Autologous MSCs can potentially be used to replace tissue defects, but the procedure also carries risks such as immunization and xenogeneic infect...

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Bibliographic Details
Published in:Stem cell research & therapy 2015-09, Vol.6 (1), p.167-167, Article 167
Main Authors: Castrén, Eeva, Sillat, Tarvo, Oja, Sofia, Noro, Ariel, Laitinen, Anita, Konttinen, Yrjö T, Lehenkari, Petri, Hukkanen, Mika, Korhonen, Matti
Format: Article
Language:English
Subjects:
Adult
Analysis
Cattle
Cell differentiation
Cells, Cultured
Collagen
Genetic aspects
Health aspects
Humans
Infection
Mesenchymal Stromal Cells - cytology
Osteoblasts - cytology
Osteogenesis
Phosphatases
Physiological aspects
Primary Cell Culture - methods
Serum
Stem cells
Tissue engineering
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Summary:Bone marrow-derived mesenchymal stromal cells (MSCs) have been intensely studied for the purpose of developing solutions for clinical tissue engineering. Autologous MSCs can potentially be used to replace tissue defects, but the procedure also carries risks such as immunization and xenogeneic infection. Replacement of the commonly used fetal calf serum (FCS) with human platelet lysate and plasma (PLP) to support cell growth may reduce some of these risks. Altered media could, however, influence stem cell differentiation and we address this experimentally. We examined human MSC differentiation into the osteoblast lineage using in vitro two- and three-dimensional cultures with PLP or FCS as cell culture medium supplements. Differentiation was followed by quantitative polymerase chain reaction, and alkaline phosphatase activity, matrix formation and matrix calcium content were quantified. Three-dimensional culture, where human MSCs were grown on collagen sponges, markedly stimulated osteoblast differentiation; a fourfold increase in calcium deposition could be observed in both PLP and FCS groups. PLP-grown cells showed robust osteogenic differentiation both in two- and three-dimensional MSC cultures. The calcium content of the matrix in the two-dimensional PLP group at day 14 was 2.2-fold higher in comparison to the FCS group (p 
ISSN:1757-6512
1757-6512
DOI:10.1186/s13287-015-0162-6