Recombinant H7 hemagglutinin forms subviral particles that protect mice and ferrets from challenge with H7N9 influenza virus

Highlights • Recombinant full-length H7 hemagglutinin (rH7) was expressed in Sf9 cells. • Purified rH7 forms subviral particles (SVP) of approximately 20 nm in diameter. • SVP structures are composed of approximately 3–4 trimers of rH7. • Purified rH7 SVP elicit protective immune response in mice an...

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Bibliographic Details
Published in:Vaccine 2015-09, Vol.33 (38), p.4975-4982
Main Authors: Pushko, Peter, Pujanauski, Lindsey M, Sun, Xiangjie, Pearce, Melissa, Hidajat, Rachmat, Kort, Thomas, Schwartzman, Louis M, Tretyakova, Irina, Chunqing, Liu, Taubenberger, Jeffery K, Tumpey, Terrence M
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Atoms & subatomic particles
Avian flu
Cloning
Disease Models, Animal
Female
Ferrets
H7N9
Hemagglutination
Hemagglutinin Glycoproteins, Influenza Virus - immunology
Immunogenicity
Infections
Influenza
Influenza A Virus, H7N9 Subtype - immunology
Influenza vaccine
Influenza Vaccines - administration & dosage
Influenza Vaccines - immunology
Influenza virus
Male
Mice, Inbred BALB C
Molecular weight
Mustela
Orthomyxoviridae Infections - prevention & control
Protein Multimerization
Proteins
Recombinant Proteins - immunology
Sf9 Cells
Spodoptera
Studies
Surfactants
Vaccines
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - immunology
Vaccines, Virus-Like Particle - administration & dosage
Vaccines, Virus-Like Particle - immunology
Virus-like particles
Viruses
VLP
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Summary:Highlights • Recombinant full-length H7 hemagglutinin (rH7) was expressed in Sf9 cells. • Purified rH7 forms subviral particles (SVP) of approximately 20 nm in diameter. • SVP structures are composed of approximately 3–4 trimers of rH7. • Purified rH7 SVP elicit protective immune response in mice and ferrets.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2015.07.026