Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli

• Published in: Biotechnology letters 2015-10, Vol.37 (10), p.2047-2053
• Main Authors: Goderska, Kamila, Juzwa, Wojciech, Szwengiel, Artur, Czarnecki, Zbigniew
• Format: Article
• Language: English
• Subjects: Acid production; Applied Microbiology; Biochemistry; Biomedical and Life Sciences; Biotechnology; Conversion; Disaccharides - metabolism; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Fermentation; Gene Expression; gene overexpression; Genes; Glucose; Lactose; Life Sciences; Microbiology; Original Research Paper; Oxidation; Oxidoreductases - genetics; Oxidoreductases - metabolism; Recombinant; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; whey; Zymomonas - enzymology; Zymomonas - genetics; Zymomonas mobilis
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-015-1887-0

Objectives To use the glucose–fructose oxidoreductase (GFOR) from Zymomonas mobilis and expressed in Escherichia coli for lactobionic acid production by conversion of lactose from whey. Results The highest concentrations of lactobionic acid (3.2 mg ml −1 ) during oxidation of whey-derived lactose by E. coli was at 24 h. Introduction of GFOR gene from Z. mobilis, into E. coli improved enzyme yields compared to what is obtainable by fermentation of the donor strain. The production of lactobionic acid by E. coli was 2.6-times higher than by Z. mobilis. Conclusions Recombinants of E. coli overexpressing the GFOR gene from Z. mobilis produced higher amount of lactobionoic acid from whey-derived lactose.