Identification of Aspergillus nomius in Bees Visiting Brazil Nut Flowers

We designed a primer pair (BtubNomF/BtubNomR) specifically for amplifying Aspergillus nomius DNA. In vitro assays confirmed BtubNomF/BtubNomR specificity, corroborating its usefulness in detecting and identifying A. nomius. We then investigated the occurrence of A. nomius in floral visitors of Berth...

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Bibliographic Details
Published in:Microbes and Environments 2015, Vol.30(3), pp.273-275
Main Authors: Massi, Fernanda Pelisson, Penha, Rafael Elias Silva, Cavalcante, Marcelo Casimiro, Viaro, Helena Paula, Silva, Josué José da, Ferranti, Larissa de Souza, Fungaro, Maria Helena Pelegrinelli
Format: Article
Language:English
Subjects:
aflatoxin
Aspergillus nomius
Aspergillus section Flavi
Bertholletia excelsa
Bombus transversalis
Centris
molecular detection
pollinators of Bertholletia excelsa
Short Communication
Xylocopa
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Summary:We designed a primer pair (BtubNomF/BtubNomR) specifically for amplifying Aspergillus nomius DNA. In vitro assays confirmed BtubNomF/BtubNomR specificity, corroborating its usefulness in detecting and identifying A. nomius. We then investigated the occurrence of A. nomius in floral visitors of Bertholletia excelsa trees by means of PCR, and A. nomius was detected in the following bees: Xylocopa frontalis, Bombus transversalis, Centris denudans, C. ferruginea, and Epicharis flava. The presence of A. nomius in bees visiting Brazil nuts opens up new avenues for obtaining novel insights into the process whereby Brazil nuts are contaminated by aflatoxin-producing fungi.
ISSN:1342-6311
1347-4405
DOI:10.1264/jsme2.ME14146