A quantitative imaging-based screen reveals the exocyst as a network hub connecting endocytosis and exocytosis

The coupling of endocytosis and exocytosis underlies fundamental biological processes ranging from fertilization to neuronal activity and cellular polarity. However, the mechanisms governing the spatial organization of endocytosis and exocytosis require clarification. Using a quantitative imaging-ba...

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Bibliographic Details
Published in:Molecular biology of the cell 2015-07, Vol.26 (13), p.2519-2534
Main Authors: Jose, Mini, Tollis, Sylvain, Nair, Deepak, Mitteau, Romain, Velours, Christophe, Massoni-Laporte, Aurelie, Royou, Anne, Sibarita, Jean-Baptiste, McCusker, Derek
Format: Article
Language:English
Subjects:
Cell Polarity - physiology
Cellular Biology
Endocytosis - physiology
Exocytosis - physiology
Life Sciences
Metabolic Networks and Pathways
Protein Transport
Saccharomyces cerevisiae Proteins - metabolism
Saccharomycetales - genetics
Saccharomycetales - metabolism
Saccharomycetales - physiology
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Summary:The coupling of endocytosis and exocytosis underlies fundamental biological processes ranging from fertilization to neuronal activity and cellular polarity. However, the mechanisms governing the spatial organization of endocytosis and exocytosis require clarification. Using a quantitative imaging-based screen in budding yeast, we identified 89 mutants displaying defects in the localization of either one or both pathways. High-resolution single-vesicle tracking revealed that the endocytic and exocytic mutants she4∆ and bud6∆ alter post-Golgi vesicle dynamics in opposite ways. The endocytic and exocytic pathways display strong interdependence during polarity establishment while being more independent during polarity maintenance. Systems analysis identified the exocyst complex as a key network hub, rich in genetic interactions with endocytic and exocytic components. Exocyst mutants displayed altered endocytic and post-Golgi vesicle dynamics and interspersed endocytic and exocytic domains compared with control cells. These data are consistent with an important role for the exocyst in coordinating endocytosis and exocytosis.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.E14-11-1527