DRC3 connects the N-DRC to dynein g to regulate flagellar waveform

The nexin-dynein regulatory complex (N-DRC), which is a major hub for the control of flagellar motility, contains at least 11 different subunits. A major challenge is to determine the location and function of each of these subunits within the N-DRC. We characterized a Chlamydomonas mutant defective...

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Bibliographic Details
Published in:Molecular biology of the cell 2015-08, Vol.26 (15), p.2788-2800
Main Authors: Awata, Junya, Song, Kangkang, Lin, Jianfeng, King, Stephen M, Sanderson, Michael J, Nicastro, Daniela, Witman, George B
Format: Article
Language:English
Subjects:
Axonemal Dyneins - metabolism
Chlamydomonas reinhardtii - genetics
Chlamydomonas reinhardtii - metabolism
Chlamydomonas reinhardtii - physiology
Cryoelectron Microscopy
Cytoskeleton - metabolism
Electron Microscope Tomography
Flagella - metabolism
Flagella - physiology
Microtubule-Associated Proteins - genetics
Microtubule-Associated Proteins - metabolism
Microtubules - metabolism
Protein Structure, Tertiary
Structure-Activity Relationship
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Summary:The nexin-dynein regulatory complex (N-DRC), which is a major hub for the control of flagellar motility, contains at least 11 different subunits. A major challenge is to determine the location and function of each of these subunits within the N-DRC. We characterized a Chlamydomonas mutant defective in the N-DRC subunit DRC3. Of the known N-DRC subunits, the drc3 mutant is missing only DRC3. Like other N-DRC mutants, the drc3 mutant has a defect in flagellar motility. However, in contrast to other mutations affecting the N-DRC, drc3 does not suppress flagellar paralysis caused by loss of radial spokes. Cryo-electron tomography revealed that the drc3 mutant lacks a portion of the N-DRC linker domain, including the L1 protrusion, part of the distal lobe, and the connection between these two structures, thus localizing DRC3 to this part of the N-DRC. This and additional considerations enable us to assign DRC3 to the L1 protrusion. Because the L1 protrusion is the only non-dynein structure in contact with the dynein g motor domain in wild-type axonemes and this is the only N-DRC-dynein connection missing in the drc3 mutant, we conclude that DRC3 interacts with dynein g to regulate flagellar waveform.
ISSN:1059-1524
1939-4586
1939-4586
DOI:10.1091/mbc.E15-01-0018