Genomic Copy Number Variations in the Myelodysplastic Syndrome and Acute Myeloid Leukemia Patients with del(5q) and/or -7/del(7q)

• Published in: International journal of medical sciences 2015-01, Vol.12 (9), p.719-726
• Main Authors: Zhang, Rui, Kim, Young-Mi, Wang, Xianfu, Li, Yan, Lu, Xianglan, Sternenberger, Andrea R, Li, Shibo, Lee, Ji-Yun
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Child; Child, Preschool; Chromosome Deletion; Chromosomes, Human, Pair 5; Chromosomes, Human, Pair 7; Comparative Genomic Hybridization; DNA Copy Number Variations; ETS Translocation Variant 6 Protein; Female; Humans; In Situ Hybridization, Fluorescence; Leukemia, Myeloid, Acute - genetics; Male; Middle Aged; Myelodysplastic Syndromes - genetics; Proto-Oncogene Proteins c-ets - genetics; Repressor Proteins - genetics; Research Paper; Tumor Suppressor Protein p53 - genetics; Young Adult
• ISSN: 1449-1907; 1449-1907
• DOI: 10.7150/ijms.12612

The most common chromosomal abnormalities in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) are -5/del(5q) and -7/del(7q). When -5/del(5q) and -7/del(7q) coexist in patients, a poor prognosis is typically associated. Given that -5/del(5q) and/or -7/del(7q) often are accompanied with additional recurrent chromosomal alterations, genetic change(s) on the accompanying chromosome(s) other than chromosomes 5 and 7 may be important factor(s) affecting leukemogenesis and disease prognosis. Using an integrated analysis of karyotype, FISH and array CGH results in this study, we evaluated the smallest region of overlap (SRO) of chromosomes 5 and 7 as well as copy number alterations (CNAs) on the other chromosomes. Moreover, the relationship between the CNAs and del(5q) and -7/del(7q) was investigated by categorizing the cases into three groups based on the abnormalities of chromosomes 5 and 7 [group I: cases only with del(5q), group II: cases only with -7/del(7q) and group III: concurrent del(5q) and del(7q) cases]. The overlapping SRO of chromosome 5 from groups I and III was 5q31.1-33.1 and of chromosome 7 from groups II and III was 7q31.31-q36.1. A total of 318 CNAs were observed; ~ 78.3% of them were identified on chromosomes other than chromosomes 5 and 7, which were defined as 'other CNAs'. Group III was a distinctive group carrying the most high number (HN) CNAs, cryptic CNAs and 'other CNAs'. The loss of TP53 was highly associated with del(5q). The loss of ETV6 was specifically associated with group III. These CNAs or genes may play a secondary role in disease progression and should be further evaluated for their clinical significance and influence on therapeutic approaches in patients with MDS/AML carrying del(5q) and/or -7/del(7q) in large-scale, patient population study.