Cloning and identification of NS5ATP2 gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A

To clone, identify and study new NS5ATP2 gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A. On the basis of subtractive cDNA library of genes transactivated by NS5A protein of hepatitis C virus, the coding sequence of new gene and its spliced variant were obt...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2004-06, Vol.10 (12), p.1735-1739
Main Authors: Yang, Qian, Cheng, Jun, Liu, Yan, Hong, Yuan, Wang, Jian-Jun, Zhang, Shu-Lin
Format: Article
Language:English
Subjects:
Antigens, Polyomavirus Transforming - genetics
Base Sequence
Cell Line, Tumor
Cloning, Molecular
Gene Expression Regulation, Viral
Gene Library
Hepacivirus - genetics
Hepatoblastoma
Humans
Liver Neoplasms
Molecular Sequence Data
Promoter Regions, Genetic
RNA Splicing
Viral Hepatitis
Viral Nonstructural Proteins - genetics
Viral Nonstructural Proteins - metabolism
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Summary:To clone, identify and study new NS5ATP2 gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A. On the basis of subtractive cDNA library of genes transactivated by NS5A protein of hepatitis C virus, the coding sequence of new gene and its spliced variant were obtained by bioinformatics method. Polymerase chain reaction (PCR) was conducted to amplify NS5ATP2 gene. The coding sequence of a new gene and its spliced variant were cloned and identified successfully. A new gene has been recognized as the new target transactivated by HCV NS5A protein. These results brought some new clues for studying the biological functions of new genes and pathogenesis of the viral proteins.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v10.i12.1735