Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes

Three multiplex PCR assays were developed to identify the 11 most common Listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups IVb, IIb, IIa, and IIc were identified accurately. This novel tool is a rapid and efficient alternative to multilocus sequence...

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Bibliographic Details
Published in:Journal of clinical microbiology 2015-10, Vol.53 (10), p.3355-3358
Main Authors: Chenal-Francisque, Viviane, Maury, Mylène M, Lavina, Morgane, Touchon, Marie, Leclercq, Alexandre, Lecuit, Marc, Brisse, Sylvain
Format: Article
Language:English
Subjects:
Bacteriology
Epidemiology
Food Microbiology
Genotype
Human health and pathology
Humans
Infectious diseases
Life Sciences
Listeria monocytogenes
Listeria monocytogenes - classification
Listeria monocytogenes - genetics
Listeria monocytogenes - isolation & purification
Listeriosis
Listeriosis - diagnosis
Listeriosis - microbiology
Microbiology and Parasitology
Molecular Typing
Molecular Typing - methods
Multiplex Polymerase Chain Reaction
Multiplex Polymerase Chain Reaction - methods
Time Factors
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Summary:Three multiplex PCR assays were developed to identify the 11 most common Listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups IVb, IIb, IIa, and IIc were identified accurately. This novel tool is a rapid and efficient alternative to multilocus sequence typing for identification of L. monocytogenes clones.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00738-15