Cyclooxygenase 2, pS2, inducible nitric oxide synthase and transforming growth factor alpha in gastric adaptation to stress

To determine the role of mucosal gene expression of cyclooxygenase 2 (COX-2), pS2 (belongs to trefoil peptides), inducible nitric oxide synthase (iNOS) and transforming growth factor alpha (TGFalpha) in gastric adaptation to water immersion and restraint stress (WRS) in rats. Wistar rats were expose...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2004-12, Vol.10 (23), p.3537-3541
Main Authors: Nie, Shi-Nan, Sun, Hai-Chen, Wu, Xue-Hao, Qian, Xiao-Ming
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Animals
Brief Reports
Cyclooxygenase 2
Gastric Mucosa - metabolism
Isoenzymes - genetics
Isoenzymes - metabolism
Male
Nitric Oxide Synthase - genetics
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type II
Prostaglandin-Endoperoxide Synthases - genetics
Prostaglandin-Endoperoxide Synthases - metabolism
Proteins - genetics
Proteins - metabolism
Rats
Rats, Wistar
Stomach - physiopathology
Stomach Ulcer - metabolism
Stomach Ulcer - physiopathology
Stress, Physiological - metabolism
Stress, Physiological - physiopathology
Transforming Growth Factor alpha - genetics
Transforming Growth Factor alpha - metabolism
Trefoil Factor-1
Tumor Suppressor Proteins
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Summary:To determine the role of mucosal gene expression of cyclooxygenase 2 (COX-2), pS2 (belongs to trefoil peptides), inducible nitric oxide synthase (iNOS) and transforming growth factor alpha (TGFalpha) in gastric adaptation to water immersion and restraint stress (WRS) in rats. Wistar rats were exposed to single or repeated WRS for 4 h every other day for up to 6 d. Gastric mucosal blood flow (GMBF) was measured by laser Doppler flowmeter-3. The extent of gastric mucosal lesions were evaluated grossly and histologically and expressions of COX-2, pS2,iNOS and TGFalpha were determined by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot. The damage to the surface of gastric epithelium with focal areas of deep haemorrhagic necrosis was induced by repeated WRS. The adaptative cytoprotection against stress was developed with activation of cell proliferation in the neck regions of gastric glands. The ulcer index (UI) in groups II, III and IV was markedly reduced as compared with group I (I: 47.23+/-1.20; IV: 10.39+/-1.18,P
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v10.i23.3537