Short‐term natural history of high‐risk human papillomavirus infection in mid‐adult women sampled monthly

• Published in: International journal of cancer 2015-11, Vol.137 (10), p.2432-2442
• Main Authors: Fu, Tsung‐chieh (Jane), Fu Xi, Long, Hulbert, Ayaka, Hughes, James P., Feng, Qinghua, Schwartz, Stephen M., Hawes, Stephen E., Koutsky, Laura A., Winer, Rachel L.
• Format: Article
• Language: English
• Subjects: Adult; Cancer; Cervical cancer; DNA, Viral - analysis; Early Diagnosis; Female; HIV; Human immunodeficiency virus; Human papillomavirus; Humans; Infections; Longitudinal Studies; Medical research; Middle Aged; Multivariate analysis; natural history; Papillomaviridae - genetics; Papillomaviridae - physiology; Papillomavirus Infections - diagnosis; Papillomavirus Infections - virology; Pregnancy; Risk Factors; Short term; Smoking; Smoking - adverse effects; Vaginal Smears - methods; Viral Load; women; Womens health
• ISSN: 0020-7136; 1097-0215
• DOI: 10.1002/ijc.29602

Characterizing short‐term HPV detection patterns and viral load may inform HPV natural history in mid‐adult women. From 2011–2012, we recruited women aged 30–50 years. Women submitted monthly self‐collected vaginal samples for high‐risk HPV DNA testing for 6 months. Positive samples were tested for type‐specific HPV DNA load by real‐time PCR. HPV type‐adjusted linear and Poisson regression assessed factors associated with (i) viral load at initial HPV detection and (ii) repeat type‐specific HPV detection. One‐hundred thirty‐nine women (36% of 387 women with ≥4 samples) contributed 243 type‐specific HR HPV infections during the study; 54% of infections were prevalent and 46% were incident. Incident (vs. prevalent) detection and past pregnancy were associated with lower viral load, whereas current smoking was associated with higher viral load. In multivariate analysis, current smoking was associated with a 40% (95% CI: 5–87%) increase in the proportion of samples that were repeatedly positive for the same HPV type, whereas incident (vs. prevalent) detection status and past pregnancy were each associated with a reduction in the proportion of samples repeatedly positive (55%, 95% CI: 38–67% and 26%, 95% CI: 10–39%, respectively). In a separate multivariate model, each log10 increase in viral load was associated with a 10% (95% CI: 4–16%) increase in the proportion of samples repeatedly positive. Factors associated with repeat HPV detection were similar to those observed in longer‐term studies, suggesting that short‐term repeat detection may relate to long‐term persistence. The negative associations between incident HPV detection and both viral load and repeat detection suggest that reactivation or intermittent persistence was more common than new acquisition. What's New? Cervical cancer arises from long‐term infection with a high‐risk strain of HPV. But when does a short‐term infection become long‐term? This study tested HPV samples from women ages 30‐50 over the course of 6 months. They tracked behavioral, clinical, and demographic factors, and found that the risk factors for repeat detection of high‐risk HPV in the short term match those for long‐term persistence, suggesting that a 6‐month infection may already be on the road to clinical significance. They also concluded that most of the HPV infections detected were pre‐existing, rather than newly acquired, so vaccination wouldn't have much benefit for this age group.