Ultra-high-resolution 3D digitalized imaging of the cerebral angioarchitecture in rats using synchrotron radiation

The angioarchitecture is a fundamental aspect of brain development and physiology. However, available imaging tools are unsuited for non-destructive cerebral mapping of the functionally important three-dimensional (3D) vascular microstructures. To address this issue, we developed an ultra-high resol...

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Bibliographic Details
Published in:Scientific reports 2015-10, Vol.5 (1), p.14982-14982, Article 14982
Main Authors: Zhang, Meng-Qi, Zhou, Luo, Deng, Qian-Fang, Xie, Yuan-Yuan, Xiao, Ti-Qiao, Cao, Yu-Ze, Zhang, Ji-Wen, Chen, Xu-Meng, Yin, Xian-Zhen, Xiao, Bo
Format: Article
Language:English
Subjects:
639/705/117
692/308/1426
Anatomy
Animal models
Animals
Brain - blood supply
Brain mapping
Brain Mapping - methods
Cerebral cortex
Cerebrovascular system
Corpus striatum
Cortex (frontal)
Data processing
Endoscopy
Humanities and Social Sciences
Image Processing, Computer-Assisted - methods
Imaging, Three-Dimensional - methods
Male
Microvasculature
Microvessels
multidisciplinary
Neostriatum
Neurodevelopmental disorders
Neuroimaging
Rats
Rats, Sprague-Dawley
Rodents
Science
Synchrotrons
Tomography, X-Ray Computed
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Summary:The angioarchitecture is a fundamental aspect of brain development and physiology. However, available imaging tools are unsuited for non-destructive cerebral mapping of the functionally important three-dimensional (3D) vascular microstructures. To address this issue, we developed an ultra-high resolution 3D digitalized angioarchitectural map for rat brain, based on synchrotron radiation phase contrast imaging (SR-PCI) with pixel size of 5.92 μm. This approach provides a systematic and detailed view of the cerebrovascular anatomy at the micrometer level without any need for contrast agents. From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum. We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D. The SR-PCI method for systematic visualization of cerebral microvasculature holds considerable promise for wider application in life sciences, including 3D micro-imaging in experimental models of neurodevelopmental and vascular disorders.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep14982