Profiling RNA editing in human tissues: towards the inosinome Atlas

Adenine to Inosine RNA editing is a widespread co- and post-transcriptional mechanism mediated by ADAR enzymes acting on double stranded RNA. It has a plethora of biological effects, appears to be particularly pervasive in humans with respect to other mammals and is implicated in a number of diverse...

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Published in:Scientific reports 2015-10, Vol.5 (1), p.14941-14941, Article 14941
Main Authors: Picardi, Ernesto, Manzari, Caterina, Mastropasqua, Francesca, Aiello, Italia, D’Erchia, Anna Maria, Pesole, Graziano
Format: Article
Language:English
Subjects:
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Adenine
Adenine - metabolism
Adenosine Deaminase - genetics
Adenosine Deaminase - metabolism
Base Sequence
Binding Sites - genetics
Biological effects
Brain - metabolism
Cancer
Cell Line
Computational neuroscience
Databases, Genetic
Double-stranded RNA
Editing
Enzymes
Genome, Human - genetics
Genomes
Humanities and Social Sciences
Humans
Inosine - metabolism
Internet
Kidney - metabolism
Liver - metabolism
Lung - metabolism
multidisciplinary
Muscles - metabolism
Myocardium - metabolism
Neurological diseases
Nucleotide sequence
Organ Specificity - genetics
Post-transcription
RNA - genetics
RNA - metabolism
RNA Editing
Science
Sequence Analysis, DNA - methods
Transcription
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Summary:Adenine to Inosine RNA editing is a widespread co- and post-transcriptional mechanism mediated by ADAR enzymes acting on double stranded RNA. It has a plethora of biological effects, appears to be particularly pervasive in humans with respect to other mammals and is implicated in a number of diverse human pathologies. Here we present the first human inosinome atlas comprising 3,041,422 A-to-I events identified in six tissues from three healthy individuals. Matched directional total-RNA-Seq and whole genome sequence datasets were generated and analysed within a dedicated computational framework, also capable of detecting hyper-edited reads. Inosinome profiles are tissue specific and edited gene sets consistently show enrichment of genes involved in neurological disorders and cancer. Overall frequency of editing also varies, but is strongly correlated with ADAR expression levels. The inosinome database is available at: http://srv00.recas.ba.infn.it/editing .
ISSN:2045-2322
2045-2322
DOI:10.1038/srep14941