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Mast cell and histamine content of human bronchoalveolar lavage fluid
Bronchoalveolar lavage was performed in 97 patients including control patients with bronchial carcinoma (24) and patients with sarcoidosis (20), cryptogenic fibrosing alveolitis (9), and asthma (4), and others. Cytocentrifuged slides were stained by two methods: May-Grünwald Giemsa and toluidine blu...
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Published in: | Thorax 1985-10, Vol.40 (10), p.760-767 |
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description | Bronchoalveolar lavage was performed in 97 patients including control patients with bronchial carcinoma (24) and patients with sarcoidosis (20), cryptogenic fibrosing alveolitis (9), and asthma (4), and others. Cytocentrifuged slides were stained by two methods: May-Grünwald Giemsa and toluidine blue. In the last 32 subjects the bronchoalveolar lavage fluid was separated into supernatant and cell pellet for the subsequent assay of the performed mast cell mediator, histamine. Comparison of the two methods of staining showed a bias towards toluidine blue. Controls had a differential mean (SE) mast cell count of 0.07% (0.01%). Higher counts were noted in cryptogenic fibrosing alveolitis--0.61% (0.15%) (p less than 0.001)--and in sarcoidosis--0.14% (0.02%) (p less than 0.05). There was a strong correlation between absolute mast cell counts and cell lysate histamine concentration (r = 0.78, p less than 0.001). Less strong, significant, correlations between supernatant histamine concentration and absolute mast cell counts (r = 0.48, p less than 0.01) or cell lysate histamine concentration (r = 0.72, p less than 0.01) were also found. Derived mean values of histamine per mast cell ranged from 3.7 to 10.9 picograms. The mean histamine content of lavage fluid supernatant as a percentage of the total lavage fluid histamine was 24.9% (3.3%). The possible clinical significance of these findings is discussed. |
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Cytocentrifuged slides were stained by two methods: May-Grünwald Giemsa and toluidine blue. In the last 32 subjects the bronchoalveolar lavage fluid was separated into supernatant and cell pellet for the subsequent assay of the performed mast cell mediator, histamine. Comparison of the two methods of staining showed a bias towards toluidine blue. Controls had a differential mean (SE) mast cell count of 0.07% (0.01%). Higher counts were noted in cryptogenic fibrosing alveolitis--0.61% (0.15%) (p less than 0.001)--and in sarcoidosis--0.14% (0.02%) (p less than 0.05). There was a strong correlation between absolute mast cell counts and cell lysate histamine concentration (r = 0.78, p less than 0.001). Less strong, significant, correlations between supernatant histamine concentration and absolute mast cell counts (r = 0.48, p less than 0.01) or cell lysate histamine concentration (r = 0.72, p less than 0.01) were also found. Derived mean values of histamine per mast cell ranged from 3.7 to 10.9 picograms. The mean histamine content of lavage fluid supernatant as a percentage of the total lavage fluid histamine was 24.9% (3.3%). The possible clinical significance of these findings is discussed.</description><identifier>ISSN: 0040-6376</identifier><identifier>EISSN: 1468-3296</identifier><identifier>DOI: 10.1136/thx.40.10.760</identifier><identifier>PMID: 4060097</identifier><identifier>CODEN: THORA7</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and British Thoracic Society</publisher><subject>Adult ; Aged ; Asthma - pathology ; Biological and medical sciences ; Bronchi - pathology ; Carcinoma, Bronchogenic - pathology ; Cell Count ; Female ; Histamine - metabolism ; Humans ; Investigative techniques of respiratory function ; Investigative techniques, diagnostic techniques (general aspects) ; Lung Diseases - pathology ; Lung Neoplasms - pathology ; Male ; Mast Cells - metabolism ; Mast Cells - pathology ; Medical sciences ; Middle Aged ; Pulmonary Alveoli - pathology ; Pulmonary Fibrosis - pathology ; Sarcoidosis - pathology ; Therapeutic Irrigation</subject><ispartof>Thorax, 1985-10, Vol.40 (10), p.760-767</ispartof><rights>1986 INIST-CNRS</rights><rights>Copyright BMJ Publishing Group LTD Oct 1985</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b509t-acd1fde9fc8083a1ea0522e8b9eb0de0a48483ceb4df8497f77f6c5c8aaf1c003</citedby><cites>FETCH-LOGICAL-b509t-acd1fde9fc8083a1ea0522e8b9eb0de0a48483ceb4df8497f77f6c5c8aaf1c003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC460180/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC460180/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8446512$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4060097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Agius, R M</creatorcontrib><creatorcontrib>Godfrey, R C</creatorcontrib><creatorcontrib>Holgate, S T</creatorcontrib><title>Mast cell and histamine content of human bronchoalveolar lavage fluid</title><title>Thorax</title><addtitle>Thorax</addtitle><description>Bronchoalveolar lavage was performed in 97 patients including control patients with bronchial carcinoma (24) and patients with sarcoidosis (20), cryptogenic fibrosing alveolitis (9), and asthma (4), and others. Cytocentrifuged slides were stained by two methods: May-Grünwald Giemsa and toluidine blue. In the last 32 subjects the bronchoalveolar lavage fluid was separated into supernatant and cell pellet for the subsequent assay of the performed mast cell mediator, histamine. Comparison of the two methods of staining showed a bias towards toluidine blue. Controls had a differential mean (SE) mast cell count of 0.07% (0.01%). Higher counts were noted in cryptogenic fibrosing alveolitis--0.61% (0.15%) (p less than 0.001)--and in sarcoidosis--0.14% (0.02%) (p less than 0.05). There was a strong correlation between absolute mast cell counts and cell lysate histamine concentration (r = 0.78, p less than 0.001). Less strong, significant, correlations between supernatant histamine concentration and absolute mast cell counts (r = 0.48, p less than 0.01) or cell lysate histamine concentration (r = 0.72, p less than 0.01) were also found. Derived mean values of histamine per mast cell ranged from 3.7 to 10.9 picograms. The mean histamine content of lavage fluid supernatant as a percentage of the total lavage fluid histamine was 24.9% (3.3%). The possible clinical significance of these findings is discussed.</description><subject>Adult</subject><subject>Aged</subject><subject>Asthma - pathology</subject><subject>Biological and medical sciences</subject><subject>Bronchi - pathology</subject><subject>Carcinoma, Bronchogenic - pathology</subject><subject>Cell Count</subject><subject>Female</subject><subject>Histamine - metabolism</subject><subject>Humans</subject><subject>Investigative techniques of respiratory function</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Lung Diseases - pathology</subject><subject>Lung Neoplasms - pathology</subject><subject>Male</subject><subject>Mast Cells - metabolism</subject><subject>Mast Cells - pathology</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Pulmonary Alveoli - pathology</subject><subject>Pulmonary Fibrosis - pathology</subject><subject>Sarcoidosis - pathology</subject><subject>Therapeutic Irrigation</subject><issn>0040-6376</issn><issn>1468-3296</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNqFkc1v1DAQxS0EKtvCkSNSJCrUS5bxxrGdA4dqKQVpKRyg4mZNHLvJktjFTlbtf4-3u1o-LpzGo_eb8Rs9Ql5QmFNa8Ddjezdn6Q1zweERmVHGZV4sKv6YzAAY5LwQ_Ck5jnENAJJScUSOGHCASszIxSeMY6ZN32fomqzt4ohD50ymvRuNGzNvs3Ya0GV18E63HvuN8T2GrMcN3pjM9lPXPCNPLPbRPN_XE_Lt_cXX5Yd89fny4_J8ldclVGOOuqG2MZXVEmSB1CCUi4WRdWVqaAwgk0wW2tSssZJVwgphuS61RLRUAxQn5O1u7-1UD6bRyWDAXt2GbsBwrzx26m_Fda268RvFOFC5nX-9nw_-52TiqIYubo9HZ_wUleCsFJUsE_jqH3Dtp-DSbYoKQQso2MO6fEfp4GMMxh6cUFDbcFQKRzHYtimcxL_80_6B3qeR9NO9jlFjbwM63cUDJhnjJV38_jaFZe4OMoYfiotClOrqeqmW776srq_4d3WZ-LMdXw_r_zj8BebOtSg</recordid><startdate>19851001</startdate><enddate>19851001</enddate><creator>Agius, R M</creator><creator>Godfrey, R C</creator><creator>Holgate, S T</creator><general>BMJ Publishing Group Ltd and British Thoracic Society</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19851001</creationdate><title>Mast cell and histamine content of human bronchoalveolar lavage fluid</title><author>Agius, R M ; Godfrey, R C ; Holgate, S T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b509t-acd1fde9fc8083a1ea0522e8b9eb0de0a48483ceb4df8497f77f6c5c8aaf1c003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Asthma - pathology</topic><topic>Biological and medical sciences</topic><topic>Bronchi - pathology</topic><topic>Carcinoma, Bronchogenic - pathology</topic><topic>Cell Count</topic><topic>Female</topic><topic>Histamine - metabolism</topic><topic>Humans</topic><topic>Investigative techniques of respiratory function</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Lung Diseases - pathology</topic><topic>Lung Neoplasms - pathology</topic><topic>Male</topic><topic>Mast Cells - metabolism</topic><topic>Mast Cells - pathology</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Pulmonary Alveoli - pathology</topic><topic>Pulmonary Fibrosis - pathology</topic><topic>Sarcoidosis - pathology</topic><topic>Therapeutic Irrigation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Agius, R M</creatorcontrib><creatorcontrib>Godfrey, R C</creatorcontrib><creatorcontrib>Holgate, S T</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Thorax</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Agius, R M</au><au>Godfrey, R C</au><au>Holgate, S T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mast cell and histamine content of human bronchoalveolar lavage fluid</atitle><jtitle>Thorax</jtitle><addtitle>Thorax</addtitle><date>1985-10-01</date><risdate>1985</risdate><volume>40</volume><issue>10</issue><spage>760</spage><epage>767</epage><pages>760-767</pages><issn>0040-6376</issn><eissn>1468-3296</eissn><coden>THORA7</coden><abstract>Bronchoalveolar lavage was performed in 97 patients including control patients with bronchial carcinoma (24) and patients with sarcoidosis (20), cryptogenic fibrosing alveolitis (9), and asthma (4), and others. Cytocentrifuged slides were stained by two methods: May-Grünwald Giemsa and toluidine blue. In the last 32 subjects the bronchoalveolar lavage fluid was separated into supernatant and cell pellet for the subsequent assay of the performed mast cell mediator, histamine. Comparison of the two methods of staining showed a bias towards toluidine blue. Controls had a differential mean (SE) mast cell count of 0.07% (0.01%). Higher counts were noted in cryptogenic fibrosing alveolitis--0.61% (0.15%) (p less than 0.001)--and in sarcoidosis--0.14% (0.02%) (p less than 0.05). There was a strong correlation between absolute mast cell counts and cell lysate histamine concentration (r = 0.78, p less than 0.001). Less strong, significant, correlations between supernatant histamine concentration and absolute mast cell counts (r = 0.48, p less than 0.01) or cell lysate histamine concentration (r = 0.72, p less than 0.01) were also found. Derived mean values of histamine per mast cell ranged from 3.7 to 10.9 picograms. The mean histamine content of lavage fluid supernatant as a percentage of the total lavage fluid histamine was 24.9% (3.3%). The possible clinical significance of these findings is discussed.</abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and British Thoracic Society</pub><pmid>4060097</pmid><doi>10.1136/thx.40.10.760</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adult Aged Asthma - pathology Biological and medical sciences Bronchi - pathology Carcinoma, Bronchogenic - pathology Cell Count Female Histamine - metabolism Humans Investigative techniques of respiratory function Investigative techniques, diagnostic techniques (general aspects) Lung Diseases - pathology Lung Neoplasms - pathology Male Mast Cells - metabolism Mast Cells - pathology Medical sciences Middle Aged Pulmonary Alveoli - pathology Pulmonary Fibrosis - pathology Sarcoidosis - pathology Therapeutic Irrigation |
title | Mast cell and histamine content of human bronchoalveolar lavage fluid |
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