Cross-Omics Comparison of Stress Responses in Mesothelial Cells Exposed to Heat- versus Filter-Sterilized Peritoneal Dialysis Fluids

• Published in: BioMed research international 2015-01, Vol.2015 (2015), p.1-12
• Main Authors: Aufricht, Christoph, Bialas, Katarzyna, Tarantino, Silvia, Herzog, Rebecca, Lichtenauer, Anton M., Bender, Thorsten O., Kratochwill, Klaus, Jörres, Achim
• Format: Article
• Language: English
• Subjects: Biomedical research; Cells, Cultured; Colleges & universities; Continuous ambulatory peritoneal dialysis; Cytotoxicity; Dialysis Solutions - adverse effects; Dialysis Solutions - chemistry; Dialysis Solutions - isolation & purification; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Epithelial Cells - pathology; Experiments; Filtration; Gene expression; Gene Expression Profiling - methods; Glucose; Heat shock proteins; Heat-Shock Proteins - genetics; Heat-Shock Proteins - metabolism; Heat-Shock Response - drug effects; Hot Temperature; Humans; Medical research; Medicine, Experimental; Oxidative stress; Peritoneal dialysis; Peritoneal Dialysis - adverse effects; Peritoneum - drug effects; Peritoneum - metabolism; Peritoneum - pathology; Physiological aspects; Protein expression; Protein folding; Proteomics; Sterilization; Stress response; Systems Integration; Two-Dimensional Difference Gel Electrophoresis - methods
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2015/628158

Recent research suggests that cytoprotective responses, such as expression of heat-shock proteins, might be inadequately induced in mesothelial cells by heat-sterilized peritoneal dialysis (PD) fluids. This study compares transcriptome data and multiple protein expression profiles for providing new insight into regulatory mechanisms. Two-dimensional difference gel electrophoresis (2D-DIGE) based proteomics and topic defined gene expression microarray-based transcriptomics techniques were used to evaluate stress responses in human omental peritoneal mesothelial cells in response to heat- or filter-sterilized PD fluids. Data from selected heat-shock proteins were validated by 2D western-blot analysis. Comparison of proteomics and transcriptomics data discriminated differentially regulated protein abundance into groups depending on correlating or noncorrelating transcripts. Inadequate abundance of several heat-shock proteins following exposure to heat-sterilized PD fluids is not reflected on the mRNA level indicating interference beyond transcriptional regulation. For the first time, this study describes evidence for posttranscriptional inadequacy of heat-shock protein expression by heat-sterilized PD fluids as a novel cytotoxic property. Cross-omics technologies introduce a novel way of understanding PDF bioincompatibility and searching for new interventions to reestablish adequate cytoprotective responses.