Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil

• Published in: BioMed research international 2015-01, Vol.2015 (2015), p.1-16
• Main Authors: Pinho, João Renato Rebello, Carrilho, Flair J., Gryschek, Ronaldo Cesar Borges, Chieffi, Pedro Paulo, Gonçalves, Elenice Messias do Nascimento, Castilho, Vera Lúcia Pagliusi, Dias-Neto, Emmanuel, Sanchez, Maria Carmen Arroyo, Pinto, Pedro Luiz Silva, Alvarado-Mora, Mónica Viviana, Espírito-Santo, Maria Cristina Carvalho, Luna, Expedito José de Albuquerque
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Brazil - epidemiology; Child; Child, Preschool; Clinical Laboratory Techniques - methods; Clinical Laboratory Techniques - statistics & numerical data; Colleges & universities; Diagnosis; Diagnosis, Laboratory; Endemic Diseases - statistics & numerical data; Female; Gastroenterology; Humans; Immunoassay - methods; Immunoassay - statistics & numerical data; Immunology; Infant; Infections; Laboratories; Male; Medicine; Methods; Middle Aged; Parasites; Parasitic diseases; Parasitology; Population Surveillance - methods; Precipitin Tests - methods; Precipitin Tests - statistics & numerical data; Prevalence; Public health; Reproducibility of Results; Risk Assessment - methods; Schistosoma; Schistosomiasis; Schistosomiasis mansoni - diagnosis; Schistosomiasis mansoni - epidemiology; Schistosomiasis mansoni - parasitology; Sensitivity and Specificity; Young Adult
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2015/135689

Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infections common in areas of low endemicity (ALEs). This study compared the Kato-Katz (KK); Hoffman, Pons, and Janer (HH); enzyme-linked immunosorbent assay- (ELISA-) IgG and ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); and qPCR techniques for schistosomiasis detection in serum and fecal samples, using the circumoval precipitin test (COPT) as reference. An epidemiological survey was conducted in a randomized sample of residents from five neighborhoods of Barra Mansa, RJ, with 610 fecal and 612 serum samples. ELISA-IgM (21.4%) showed the highest positivity and HH and KK techniques were the least sensitive (0.8%). All techniques except qPCR-serum showed high accuracy (82–95.5%), differed significantly from COPT in positivity P