Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil

Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infecti...

Full description

Saved in:
Bibliographic Details
Published in:BioMed research international 2015-01, Vol.2015 (2015), p.1-16
Main Authors: Pinho, João Renato Rebello, Carrilho, Flair J., Gryschek, Ronaldo Cesar Borges, Chieffi, Pedro Paulo, Gonçalves, Elenice Messias do Nascimento, Castilho, Vera Lúcia Pagliusi, Dias-Neto, Emmanuel, Sanchez, Maria Carmen Arroyo, Pinto, Pedro Luiz Silva, Alvarado-Mora, Mónica Viviana, Espírito-Santo, Maria Cristina Carvalho, Luna, Expedito José de Albuquerque
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Brazil - epidemiology
Child
Child, Preschool
Clinical Laboratory Techniques - methods
Clinical Laboratory Techniques - statistics & numerical data
Colleges & universities
Diagnosis
Diagnosis, Laboratory
Endemic Diseases - statistics & numerical data
Female
Gastroenterology
Humans
Immunoassay - methods
Immunoassay - statistics & numerical data
Immunology
Infant
Infections
Laboratories
Male
Medicine
Methods
Middle Aged
Parasites
Parasitic diseases
Parasitology
Population Surveillance - methods
Precipitin Tests - methods
Precipitin Tests - statistics & numerical data
Prevalence
Public health
Reproducibility of Results
Risk Assessment - methods
Schistosoma
Schistosomiasis
Schistosomiasis mansoni - diagnosis
Schistosomiasis mansoni - epidemiology
Schistosomiasis mansoni - parasitology
Sensitivity and Specificity
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c598t-32a26daa1d10d4e240bcdb61e3cf15587ff52deb13defb5a10defd23551944f63
cites cdi_FETCH-LOGICAL-c598t-32a26daa1d10d4e240bcdb61e3cf15587ff52deb13defb5a10defd23551944f63
container_end_page 16
container_issue 2015
container_start_page 1
container_title BioMed research international
container_volume 2015
creator Pinho, João Renato Rebello
Carrilho, Flair J.
Gryschek, Ronaldo Cesar Borges
Chieffi, Pedro Paulo
Gonçalves, Elenice Messias do Nascimento
Castilho, Vera Lúcia Pagliusi
Dias-Neto, Emmanuel
Sanchez, Maria Carmen Arroyo
Pinto, Pedro Luiz Silva
Alvarado-Mora, Mónica Viviana
Espírito-Santo, Maria Cristina Carvalho
Luna, Expedito José de Albuquerque
description Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infections common in areas of low endemicity (ALEs). This study compared the Kato-Katz (KK); Hoffman, Pons, and Janer (HH); enzyme-linked immunosorbent assay- (ELISA-) IgG and ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); and qPCR techniques for schistosomiasis detection in serum and fecal samples, using the circumoval precipitin test (COPT) as reference. An epidemiological survey was conducted in a randomized sample of residents from five neighborhoods of Barra Mansa, RJ, with 610 fecal and 612 serum samples. ELISA-IgM (21.4%) showed the highest positivity and HH and KK techniques were the least sensitive (0.8%). All techniques except qPCR-serum showed high accuracy (82–95.5%), differed significantly from COPT in positivity P
doi_str_mv 10.1155/2015/135689
format article
fullrecord <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4609343</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A458160212</galeid><sourcerecordid>A458160212</sourcerecordid><originalsourceid>FETCH-LOGICAL-c598t-32a26daa1d10d4e240bcdb61e3cf15587ff52deb13defb5a10defd23551944f63</originalsourceid><addsrcrecordid>eNqNkktv1DAUhSMEolXpij2yxAZBh8avPDaVpkN5aRASLWvrxo-Jq8Qe7KTV8OP4bTgzZSis6o3t689H9x6dLHuO87cYc35KcsxPMeVFVT_KDgnFbFZghh_vz5QeZMcxXudpVbjI6-JpdkAKnrOyLA-zXwvfryHAYG80uhxGtUHeoKHVaC7lGEBu7--sMTpoN6ArLVtnf4w6IuPDFlxC45OAD5vEwcr5aOP06VK2Ng4--t7CVPoCLnpnkXVoHjRsmaW_RRdO6d5KO2ymp3MIAbYsoEWqnaBv1iOl0Wdw2gafmoRBn6DzAD9t9yx7YqCL-vhuP8q-v7-4WnycLb9--LSYL2eS19UwowRIoQCwwrlimrC8kaopsKbSJBer0hhOlG4wVdo0HBKljSKUc1wzZgp6lJ3tdNdj02slkxUBOrEOtoewER6s-PfF2Vas_I1gyXHKaBJ4dScQ_OTeIHobpe66NJUfo8AlrwgrCsIfgJKyrmnO8oS-_A-99mNwyYmJIlXqn-C_1Ao6LawzPrUoJ1ExZ3wKBcEkUW92lAw-xqDNfjqciylrYsqa2GUt0S_uG7Jn_yQrAa93QGudglv7MDWdEG3gHswZxQX9DUuc5og</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1722855121</pqid></control><display><type>article</type><title>Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil</title><source>Wiley Online Library Open Access</source><source>ProQuest - Publicly Available Content Database</source><creator>Pinho, João Renato Rebello ; Carrilho, Flair J. ; Gryschek, Ronaldo Cesar Borges ; Chieffi, Pedro Paulo ; Gonçalves, Elenice Messias do Nascimento ; Castilho, Vera Lúcia Pagliusi ; Dias-Neto, Emmanuel ; Sanchez, Maria Carmen Arroyo ; Pinto, Pedro Luiz Silva ; Alvarado-Mora, Mónica Viviana ; Espírito-Santo, Maria Cristina Carvalho ; Luna, Expedito José de Albuquerque</creator><contributor>Karl, Stephan</contributor><creatorcontrib>Pinho, João Renato Rebello ; Carrilho, Flair J. ; Gryschek, Ronaldo Cesar Borges ; Chieffi, Pedro Paulo ; Gonçalves, Elenice Messias do Nascimento ; Castilho, Vera Lúcia Pagliusi ; Dias-Neto, Emmanuel ; Sanchez, Maria Carmen Arroyo ; Pinto, Pedro Luiz Silva ; Alvarado-Mora, Mónica Viviana ; Espírito-Santo, Maria Cristina Carvalho ; Luna, Expedito José de Albuquerque ; Karl, Stephan</creatorcontrib><description>Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infections common in areas of low endemicity (ALEs). This study compared the Kato-Katz (KK); Hoffman, Pons, and Janer (HH); enzyme-linked immunosorbent assay- (ELISA-) IgG and ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); and qPCR techniques for schistosomiasis detection in serum and fecal samples, using the circumoval precipitin test (COPT) as reference. An epidemiological survey was conducted in a randomized sample of residents from five neighborhoods of Barra Mansa, RJ, with 610 fecal and 612 serum samples. ELISA-IgM (21.4%) showed the highest positivity and HH and KK techniques were the least sensitive (0.8%). All techniques except qPCR-serum showed high accuracy (82–95.5%), differed significantly from COPT in positivity P&lt;0.05, and showed poor agreement with COPT. Medium agreement was seen with ELISA-IgG (Kappa = 0.377) and IFA (Kappa = 0.347). Parasitological techniques showed much lower positivity rates than those by other techniques. We suggest the possibility of using a combination of laboratory tools for the diagnosis of schistosomiasis in ALEs.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2015/135689</identifier><identifier>PMID: 26504777</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Brazil - epidemiology ; Child ; Child, Preschool ; Clinical Laboratory Techniques - methods ; Clinical Laboratory Techniques - statistics &amp; numerical data ; Colleges &amp; universities ; Diagnosis ; Diagnosis, Laboratory ; Endemic Diseases - statistics &amp; numerical data ; Female ; Gastroenterology ; Humans ; Immunoassay - methods ; Immunoassay - statistics &amp; numerical data ; Immunology ; Infant ; Infections ; Laboratories ; Male ; Medicine ; Methods ; Middle Aged ; Parasites ; Parasitic diseases ; Parasitology ; Population Surveillance - methods ; Precipitin Tests - methods ; Precipitin Tests - statistics &amp; numerical data ; Prevalence ; Public health ; Reproducibility of Results ; Risk Assessment - methods ; Schistosoma ; Schistosomiasis ; Schistosomiasis mansoni - diagnosis ; Schistosomiasis mansoni - epidemiology ; Schistosomiasis mansoni - parasitology ; Sensitivity and Specificity ; Young Adult</subject><ispartof>BioMed research international, 2015-01, Vol.2015 (2015), p.1-16</ispartof><rights>Copyright © 2015 Maria Cristina Carvalho Espírito-Santo et al.</rights><rights>COPYRIGHT 2015 John Wiley &amp; Sons, Inc.</rights><rights>Copyright © 2015 Maria Cristina Carvalho Espirito-Santo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2015 Maria Cristina Carvalho Espírito-Santo et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c598t-32a26daa1d10d4e240bcdb61e3cf15587ff52deb13defb5a10defd23551944f63</citedby><cites>FETCH-LOGICAL-c598t-32a26daa1d10d4e240bcdb61e3cf15587ff52deb13defb5a10defd23551944f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1722855121/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1722855121?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,25753,27924,27925,37012,37013,44590,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26504777$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Karl, Stephan</contributor><creatorcontrib>Pinho, João Renato Rebello</creatorcontrib><creatorcontrib>Carrilho, Flair J.</creatorcontrib><creatorcontrib>Gryschek, Ronaldo Cesar Borges</creatorcontrib><creatorcontrib>Chieffi, Pedro Paulo</creatorcontrib><creatorcontrib>Gonçalves, Elenice Messias do Nascimento</creatorcontrib><creatorcontrib>Castilho, Vera Lúcia Pagliusi</creatorcontrib><creatorcontrib>Dias-Neto, Emmanuel</creatorcontrib><creatorcontrib>Sanchez, Maria Carmen Arroyo</creatorcontrib><creatorcontrib>Pinto, Pedro Luiz Silva</creatorcontrib><creatorcontrib>Alvarado-Mora, Mónica Viviana</creatorcontrib><creatorcontrib>Espírito-Santo, Maria Cristina Carvalho</creatorcontrib><creatorcontrib>Luna, Expedito José de Albuquerque</creatorcontrib><title>Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infections common in areas of low endemicity (ALEs). This study compared the Kato-Katz (KK); Hoffman, Pons, and Janer (HH); enzyme-linked immunosorbent assay- (ELISA-) IgG and ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); and qPCR techniques for schistosomiasis detection in serum and fecal samples, using the circumoval precipitin test (COPT) as reference. An epidemiological survey was conducted in a randomized sample of residents from five neighborhoods of Barra Mansa, RJ, with 610 fecal and 612 serum samples. ELISA-IgM (21.4%) showed the highest positivity and HH and KK techniques were the least sensitive (0.8%). All techniques except qPCR-serum showed high accuracy (82–95.5%), differed significantly from COPT in positivity P&lt;0.05, and showed poor agreement with COPT. Medium agreement was seen with ELISA-IgG (Kappa = 0.377) and IFA (Kappa = 0.347). Parasitological techniques showed much lower positivity rates than those by other techniques. We suggest the possibility of using a combination of laboratory tools for the diagnosis of schistosomiasis in ALEs.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Brazil - epidemiology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Clinical Laboratory Techniques - methods</subject><subject>Clinical Laboratory Techniques - statistics &amp; numerical data</subject><subject>Colleges &amp; universities</subject><subject>Diagnosis</subject><subject>Diagnosis, Laboratory</subject><subject>Endemic Diseases - statistics &amp; numerical data</subject><subject>Female</subject><subject>Gastroenterology</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Immunoassay - statistics &amp; numerical data</subject><subject>Immunology</subject><subject>Infant</subject><subject>Infections</subject><subject>Laboratories</subject><subject>Male</subject><subject>Medicine</subject><subject>Methods</subject><subject>Middle Aged</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Parasitology</subject><subject>Population Surveillance - methods</subject><subject>Precipitin Tests - methods</subject><subject>Precipitin Tests - statistics &amp; numerical data</subject><subject>Prevalence</subject><subject>Public health</subject><subject>Reproducibility of Results</subject><subject>Risk Assessment - methods</subject><subject>Schistosoma</subject><subject>Schistosomiasis</subject><subject>Schistosomiasis mansoni - diagnosis</subject><subject>Schistosomiasis mansoni - epidemiology</subject><subject>Schistosomiasis mansoni - parasitology</subject><subject>Sensitivity and Specificity</subject><subject>Young Adult</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqNkktv1DAUhSMEolXpij2yxAZBh8avPDaVpkN5aRASLWvrxo-Jq8Qe7KTV8OP4bTgzZSis6o3t689H9x6dLHuO87cYc35KcsxPMeVFVT_KDgnFbFZghh_vz5QeZMcxXudpVbjI6-JpdkAKnrOyLA-zXwvfryHAYG80uhxGtUHeoKHVaC7lGEBu7--sMTpoN6ArLVtnf4w6IuPDFlxC45OAD5vEwcr5aOP06VK2Ng4--t7CVPoCLnpnkXVoHjRsmaW_RRdO6d5KO2ymp3MIAbYsoEWqnaBv1iOl0Wdw2gafmoRBn6DzAD9t9yx7YqCL-vhuP8q-v7-4WnycLb9--LSYL2eS19UwowRIoQCwwrlimrC8kaopsKbSJBer0hhOlG4wVdo0HBKljSKUc1wzZgp6lJ3tdNdj02slkxUBOrEOtoewER6s-PfF2Vas_I1gyXHKaBJ4dScQ_OTeIHobpe66NJUfo8AlrwgrCsIfgJKyrmnO8oS-_A-99mNwyYmJIlXqn-C_1Ao6LawzPrUoJ1ExZ3wKBcEkUW92lAw-xqDNfjqciylrYsqa2GUt0S_uG7Jn_yQrAa93QGudglv7MDWdEG3gHswZxQX9DUuc5og</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Pinho, João Renato Rebello</creator><creator>Carrilho, Flair J.</creator><creator>Gryschek, Ronaldo Cesar Borges</creator><creator>Chieffi, Pedro Paulo</creator><creator>Gonçalves, Elenice Messias do Nascimento</creator><creator>Castilho, Vera Lúcia Pagliusi</creator><creator>Dias-Neto, Emmanuel</creator><creator>Sanchez, Maria Carmen Arroyo</creator><creator>Pinto, Pedro Luiz Silva</creator><creator>Alvarado-Mora, Mónica Viviana</creator><creator>Espírito-Santo, Maria Cristina Carvalho</creator><creator>Luna, Expedito José de Albuquerque</creator><general>Hindawi Publishing Corporation</general><general>John Wiley &amp; Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150101</creationdate><title>Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil</title><author>Pinho, João Renato Rebello ; Carrilho, Flair J. ; Gryschek, Ronaldo Cesar Borges ; Chieffi, Pedro Paulo ; Gonçalves, Elenice Messias do Nascimento ; Castilho, Vera Lúcia Pagliusi ; Dias-Neto, Emmanuel ; Sanchez, Maria Carmen Arroyo ; Pinto, Pedro Luiz Silva ; Alvarado-Mora, Mónica Viviana ; Espírito-Santo, Maria Cristina Carvalho ; Luna, Expedito José de Albuquerque</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c598t-32a26daa1d10d4e240bcdb61e3cf15587ff52deb13defb5a10defd23551944f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Brazil - epidemiology</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Clinical Laboratory Techniques - methods</topic><topic>Clinical Laboratory Techniques - statistics &amp; numerical data</topic><topic>Colleges &amp; universities</topic><topic>Diagnosis</topic><topic>Diagnosis, Laboratory</topic><topic>Endemic Diseases - statistics &amp; numerical data</topic><topic>Female</topic><topic>Gastroenterology</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Immunoassay - statistics &amp; numerical data</topic><topic>Immunology</topic><topic>Infant</topic><topic>Infections</topic><topic>Laboratories</topic><topic>Male</topic><topic>Medicine</topic><topic>Methods</topic><topic>Middle Aged</topic><topic>Parasites</topic><topic>Parasitic diseases</topic><topic>Parasitology</topic><topic>Population Surveillance - methods</topic><topic>Precipitin Tests - methods</topic><topic>Precipitin Tests - statistics &amp; numerical data</topic><topic>Prevalence</topic><topic>Public health</topic><topic>Reproducibility of Results</topic><topic>Risk Assessment - methods</topic><topic>Schistosoma</topic><topic>Schistosomiasis</topic><topic>Schistosomiasis mansoni - diagnosis</topic><topic>Schistosomiasis mansoni - epidemiology</topic><topic>Schistosomiasis mansoni - parasitology</topic><topic>Sensitivity and Specificity</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pinho, João Renato Rebello</creatorcontrib><creatorcontrib>Carrilho, Flair J.</creatorcontrib><creatorcontrib>Gryschek, Ronaldo Cesar Borges</creatorcontrib><creatorcontrib>Chieffi, Pedro Paulo</creatorcontrib><creatorcontrib>Gonçalves, Elenice Messias do Nascimento</creatorcontrib><creatorcontrib>Castilho, Vera Lúcia Pagliusi</creatorcontrib><creatorcontrib>Dias-Neto, Emmanuel</creatorcontrib><creatorcontrib>Sanchez, Maria Carmen Arroyo</creatorcontrib><creatorcontrib>Pinto, Pedro Luiz Silva</creatorcontrib><creatorcontrib>Alvarado-Mora, Mónica Viviana</creatorcontrib><creatorcontrib>Espírito-Santo, Maria Cristina Carvalho</creatorcontrib><creatorcontrib>Luna, Expedito José de Albuquerque</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East &amp; Africa Database</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest - Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pinho, João Renato Rebello</au><au>Carrilho, Flair J.</au><au>Gryschek, Ronaldo Cesar Borges</au><au>Chieffi, Pedro Paulo</au><au>Gonçalves, Elenice Messias do Nascimento</au><au>Castilho, Vera Lúcia Pagliusi</au><au>Dias-Neto, Emmanuel</au><au>Sanchez, Maria Carmen Arroyo</au><au>Pinto, Pedro Luiz Silva</au><au>Alvarado-Mora, Mónica Viviana</au><au>Espírito-Santo, Maria Cristina Carvalho</au><au>Luna, Expedito José de Albuquerque</au><au>Karl, Stephan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>2015</volume><issue>2015</issue><spage>1</spage><epage>16</epage><pages>1-16</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infections common in areas of low endemicity (ALEs). This study compared the Kato-Katz (KK); Hoffman, Pons, and Janer (HH); enzyme-linked immunosorbent assay- (ELISA-) IgG and ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); and qPCR techniques for schistosomiasis detection in serum and fecal samples, using the circumoval precipitin test (COPT) as reference. An epidemiological survey was conducted in a randomized sample of residents from five neighborhoods of Barra Mansa, RJ, with 610 fecal and 612 serum samples. ELISA-IgM (21.4%) showed the highest positivity and HH and KK techniques were the least sensitive (0.8%). All techniques except qPCR-serum showed high accuracy (82–95.5%), differed significantly from COPT in positivity P&lt;0.05, and showed poor agreement with COPT. Medium agreement was seen with ELISA-IgG (Kappa = 0.377) and IFA (Kappa = 0.347). Parasitological techniques showed much lower positivity rates than those by other techniques. We suggest the possibility of using a combination of laboratory tools for the diagnosis of schistosomiasis in ALEs.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>26504777</pmid><doi>10.1155/2015/135689</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2314-6133
ispartof BioMed research international, 2015-01, Vol.2015 (2015), p.1-16
issn 2314-6133
2314-6141
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4609343
source Wiley Online Library Open Access; ProQuest - Publicly Available Content Database
subjects Adolescent
Adult
Aged
Aged, 80 and over
Brazil - epidemiology
Child
Child, Preschool
Clinical Laboratory Techniques - methods
Clinical Laboratory Techniques - statistics & numerical data
Colleges & universities
Diagnosis
Diagnosis, Laboratory
Endemic Diseases - statistics & numerical data
Female
Gastroenterology
Humans
Immunoassay - methods
Immunoassay - statistics & numerical data
Immunology
Infant
Infections
Laboratories
Male
Medicine
Methods
Middle Aged
Parasites
Parasitic diseases
Parasitology
Population Surveillance - methods
Precipitin Tests - methods
Precipitin Tests - statistics & numerical data
Prevalence
Public health
Reproducibility of Results
Risk Assessment - methods
Schistosoma
Schistosomiasis
Schistosomiasis mansoni - diagnosis
Schistosomiasis mansoni - epidemiology
Schistosomiasis mansoni - parasitology
Sensitivity and Specificity
Young Adult
title Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T02%3A09%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20Study%20of%20the%20Accuracy%20of%20Different%20Techniques%20for%20the%20Laboratory%20Diagnosis%20of%20Schistosomiasis%20Mansoni%20in%20Areas%20of%20Low%20Endemicity%20in%20Barra%20Mansa%20City,%20Rio%20de%20Janeiro%20State,%20Brazil&rft.jtitle=BioMed%20research%20international&rft.au=Pinho,%20Jo%C3%A3o%20Renato%20Rebello&rft.date=2015-01-01&rft.volume=2015&rft.issue=2015&rft.spage=1&rft.epage=16&rft.pages=1-16&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2015/135689&rft_dat=%3Cgale_pubme%3EA458160212%3C/gale_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c598t-32a26daa1d10d4e240bcdb61e3cf15587ff52deb13defb5a10defd23551944f63%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1722855121&rft_id=info:pmid/26504777&rft_galeid=A458160212&rfr_iscdi=true