Molecular Assay for Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae Isolates from Cultures and Clinical Nucleic Acid Amplification Test Specimens

We developed a real-time PCR assay to detect single nucleotide polymorphisms associated with ciprofloxacin resistance in specimens submitted for nucleic acid amplification testing (NAAT). All three single nucleotide polymorphism (SNP) targets produced high sensitivity and specificity values. The pre...

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Bibliographic Details
Published in:Journal of clinical microbiology 2015-11, Vol.53 (11), p.3606-3608
Main Authors: Peterson, S W, Martin, I, Demczuk, W, Bharat, A, Hoang, L, Wylie, J, Allen, V, Lefebvre, B, Tyrrell, G, Horsman, G, Haldane, D, Garceau, R, Wong, T, Mulvey, M R
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Bacteriology
Canada
Ciprofloxacin - pharmacology
Cross Reactions
DNA Gyrase - genetics
DNA Topoisomerase IV - genetics
Drug Resistance, Bacterial - genetics
Gonorrhea - diagnosis
Gonorrhea - microbiology
Humans
Microbial Sensitivity Tests
Neisseria gonorrhoeae
Neisseria gonorrhoeae - drug effects
Neisseria gonorrhoeae - genetics
Neisseria gonorrhoeae - isolation & purification
Nucleic Acid Amplification Techniques - methods
Polymerase Chain Reaction - methods
Polymorphism, Single Nucleotide - genetics
Sensitivity and Specificity
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Description
Summary:We developed a real-time PCR assay to detect single nucleotide polymorphisms associated with ciprofloxacin resistance in specimens submitted for nucleic acid amplification testing (NAAT). All three single nucleotide polymorphism (SNP) targets produced high sensitivity and specificity values. The presence of ≥2 SNPs was sufficient to predict ciprofloxacin resistance in an organism.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01632-15