Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons

Neurotrophic tyrosine kinase type 1 (NTRK1) plays critical roles in proliferation, differentiation, and survival of cholinergic neurons; however, it remains unknown whether enhanced expression of NTRK1 in neural stem cells (NSCs) can promote their differentiation into mature neurons. In this study,...

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Bibliographic Details
Published in:BioMed research international 2015-01, Vol.2015 (2015), p.1-6
Main Authors: Xiao, Songhua, Lv, Ruiyan, Zhong, Zhuoyuan, He, Feng, Wang, Limin, Liu, Zhonglin
Format: Article
Language:English
Subjects:
Alzheimer's disease
Animals
Cell culture
Cell Differentiation - genetics
Cholinergic Neurons - metabolism
Gene Expression Regulation, Developmental
Genes
Kinases
Mice
Nerve Growth Factor - pharmacology
Neural Stem Cells - cytology
Neural Stem Cells - metabolism
Neurons
Neurotrophic functions
Phosphotransferases
Physiological aspects
Rats
Receptor, trkA - biosynthesis
Receptor, trkA - genetics
Stem cells
Tyrosine
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Summary:Neurotrophic tyrosine kinase type 1 (NTRK1) plays critical roles in proliferation, differentiation, and survival of cholinergic neurons; however, it remains unknown whether enhanced expression of NTRK1 in neural stem cells (NSCs) can promote their differentiation into mature neurons. In this study, a plasmid encoding the rat NTRK1 gene was constructed and transfected into C17.2 mouse neural stem cells (NSCs). NTRK1 overexpression in C17.2 cells was confirmed by western blot. The NSCs overexpressing NTRK1 and the C17.2 NSCs transfected by an empty plasmid vector were treated with or without 100 ng/mL nerve growth factor (NGF) for 7 days. Expression of the cholinergic cell marker, choline acetyltransferase (ChAT), was detected by florescent immunocytochemistry (ICC). In the presence of NGF induction, the NSCs overexpressing NTRK1 differentiated into ChAT-immunopositive cells at 3-fold higher than the NSCs transfected by the plasmid vector (26% versus 9%, P < 0.05 ). The data suggest that elevated NTRK1 expression increases differentiation of NSCs into cholinergic neurons under stimulation of NGF. The approach also represents an efficient strategy for generation of cholinergic neurons.
ISSN:2314-6133
2314-6141
DOI:10.1155/2015/857202