Effects of neurotrophins on gastrointestinal myoelectric activities of rats

• Published in: World journal of gastroenterology : WJG 2003-08, Vol.9 (8), p.1874-1877
• Main Authors: Chai, Ning-Li, Dong, Lei, Li, Zong-Fang, Du, Ke-Xin, Wang, Jian-Hua, Yan, Li-Kun, Dong, Xi-Lin
• Format: Article
• Language: English
• Subjects: Animals; Brain-Derived Neurotrophic Factor - pharmacology; Brief Reports; Digestive System - drug effects; Digestive System Physiological Phenomena; Electromyography; Female; Humans; Male; Mice; Myoelectric Complex, Migrating - drug effects; Nerve Growth Factor - pharmacology; Neurotrophin 3 - pharmacology; Rats; Rats, Sprague-Dawley; Recombinant Proteins - pharmacology; 神经生长因子; 肌电活性; 肠; 胃; 重组脑源性神经营养因子
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v9.i8.1874

AIM: To observe the effects of mouse nerve growth factor(NGF), rat recombinant brain derived neurotrophic factor(rm-BDNF) and recombinant human neurotrophin-3 (rh-NT-3) on the gastrointestinal motility and the migrating myoelectric complex (MMC) in rat.METHODS: A randomized, double-blinded, placebo-controlled experiment was performed. 5-7 days after we chronically implanted four or five bipolar silver electrodes on the stomach, duodenum, jejunum and colon, 21 experimental rats were coded and divided into 3 groups and injected NGF, rm-BDNF, rh-NT-3 or placebo respectively via tail vein at a dose of 20μg·kg^-1. The gastrointestinal myoelectrical activity was recorded 2 hours before and after the test substance infusions in these consciously fasting rats.RESULTS: The neurotrophins-induced pattern of activity was characterized by enhanced spiking activity of different amplitudes at all recording sites, especially in the colon. In the gastric antrum and intestine, only rh-NT-3 had increased effects on the demographic characteristics of electrical activities (P