Expression of insulin-like growth factor 1 and insulin-like growth factor 1 receptor and its intervention by interleukin-10 in experimental hepatic fibrosis

• Published in: World journal of gastroenterology : WJG 2003-06, Vol.9 (6), p.1287-1291
• Main Authors: Wang, Xiao-Zhong, Chen, Zhi-Xin, Zhang, Li-Juan, Chen, Yun-Xin, Li, Dan, Chen, Feng-Lin, Huang, Yue-Hong
• Format: Article
• Language: English
• Subjects: Animals; Basic Research; Immunohistochemistry; Insulin-Like Growth Factor I - antagonists & inhibitors; Insulin-Like Growth Factor I - metabolism; Interleukin-10 - pharmacology; Liver Cirrhosis, Experimental - metabolism; Male; Rats; Rats, Sprague-Dawley; Receptor, IGF Type 1 - antagonists & inhibitors; Receptor, IGF Type 1 - metabolism
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v9.i6.1287

To study the expression of IGF-1 and IGF-1R and its intervention by interleukin-10 in the course of experimental hepatic fibrosis. Hepatic fibrosis was induced in rats by carbon tetrachloride intoxication and liver specimens were taken from the rats administered CCl4 with or without IL-10 treatment and the animals of the control group. Immunoreactivities for insulin-like growth factor-1 (IGF-1) and IGF-1 receptor(IGF-1R) were demonstrated by immunohistochemistry, and their intensities were evaluated in different animal groups. The positive levels for IGF-1 and IGF-1R were increased with the development of hepatic fibrosis, with the positive signals localized in cytoplasm and/or at the plasmic membrane of hepatocytes. The positive signals of IGF-1 and IGF-1R were observed more frequently (P