SHCBP1 is required for midbody organization and cytokinesis completion

The centralspindlin complex, which is composed of MKLP1 and MgcRacGAP, is one of the crucial factors involved in cytokinesis initiation. Centralspindlin is localized at the middle of the central spindle during anaphase and then concentrates at the midbody to control abscission. A number of proteins...

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Bibliographic Details
Published in:Cell cycle (Georgetown, Tex.) Tex.), 2014-09, Vol.13 (17), p.2744-2751
Main Authors: Asano, Eri, Hasegawa, Hitoki, Hyodo, Toshinori, Ito, Satoko, Maeda, Masao, Chen, Dan, Takahashi, Masahide, Hamaguchi, Michinari, Senga, Takeshi
Format: Article
Language:English
Subjects:
abscission
centralspindlin
Cytokinesis
HeLa Cells
Humans
MgcRacGAP
midbody
Mitosis
MKLP1
Models, Biological
Protein Binding
Protein Transport
Proteolysis
RNA, Small Interfering - metabolism
Shc Signaling Adaptor Proteins - metabolism
SHCBP1
Spindle Apparatus - metabolism
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Summary:The centralspindlin complex, which is composed of MKLP1 and MgcRacGAP, is one of the crucial factors involved in cytokinesis initiation. Centralspindlin is localized at the middle of the central spindle during anaphase and then concentrates at the midbody to control abscission. A number of proteins that associate with centralspindlin have been identified. These associating factors regulate furrowing and abscission in coordination with centralspindlin. A recent study identified a novel centralspindlin partner, called Nessun Dorma, which is essential for germ cell cytokinesis in Drosophila melanogaster. SHCBP1 is a human ortholog of Nessun Dorma that associates with human centralspindlin. In this report, we analyzed the interaction of SHCBP1 with centralspindlin in detail and determined the regions that are required for the interaction. In addition, we demonstrate that the central region is necessary for the SHCBP1 dimerization. Both MgcRacGAP and MKLP1 are degraded once cells exit mitosis. Similarly, endogenous and exogenous SHCBP1 were degraded with mitosis progression. Interestingly, SHCBP1 expression was significantly reduced in the absence of centralspindlin, whereas centralspindlin expression was not affected by SHCBP1 knockdown. Finally, we demonstrate that SHCBP1 depletion promotes midbody structure disruption and inhibits abscission, a final stage of cytokinesis. Our study gives novel insight into the role of SHCBP in cytokinesis completion.
ISSN:1538-4101
1551-4005
DOI:10.4161/15384101.2015.945840