Cyclin B3 controls anaphase onset independent of spindle assembly checkpoint in meiotic oocytes

Cyclin B3 is a relatively new member of the cyclin family whose functions are little known. We found that depletion of cyclin B3 inhibited metaphase-anaphase transition as indicated by a well-sustained MI spindle and cyclin B1 expression in meiotic oocytes after extended culture. This effect was ind...

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Bibliographic Details
Published in:Cell cycle (Georgetown, Tex.) Tex.), 2015-01, Vol.14 (16), p.2648-2654
Main Authors: Zhang, Teng, Qi, Shu-Tao, Huang, Lin, Ma, Xue-Shan, Ouyang, Ying-Chun, Hou, Yi, Shen, Wei, Schatten, Heide, Sun, Qing-Yuan
Format: Article
Language:English
Subjects:
Anaphase
Animals
Cells, Cultured
Cyclin B - physiology
Female
M Phase Cell Cycle Checkpoints
Meiosis
Mice, Inbred ICR
Oocytes - physiology
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Summary:Cyclin B3 is a relatively new member of the cyclin family whose functions are little known. We found that depletion of cyclin B3 inhibited metaphase-anaphase transition as indicated by a well-sustained MI spindle and cyclin B1 expression in meiotic oocytes after extended culture. This effect was independent of spindle assembly checkpoint activity, since both Bub3 and BubR1 signals were not observed at kinetochores in MI-arrested cells. The metaphase I arrest was not rescued by either Mad2 knockdown or cdc20 overexpression, but it was rescued by securin RNAi. We conclude that cyclin B3 controls the metaphase-anaphase transition by activating APC/C(cdc20) in meiotic oocytes, a process that does not rely on SAC activity.
ISSN:1538-4101
1551-4005
DOI:10.1080/15384101.2015.1064567