In Vitro Behavior of Human Adipose Tissue-Derived Stem Cells on Poly(ε-caprolactone) Film for Bone Tissue Engineering Applications

Bone tissue engineering is an emerging field, representing one of the most exciting challenges for scientists and clinicians. The possibility of combining mesenchymal stem cells and scaffolds to create engineered tissues has brought attention to a large variety of biomaterials in combination with os...

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Bibliographic Details
Published in:BioMed research international 2015-01, Vol.2015 (2015), p.1-12
Main Authors: Brandi, Maria Luisa, Tanini, Annalisa, Chiellini, Federica, Pirosa, Alessandro, Puppi, Dario, Galli, Gianna, Zonefrati, Roberto, Romagnoli, Cecilia, Martelli, Francesco Saverio
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Adipose tissues
Body fat
Bone marrow
Bones
Cell Line
Cell Physiological Phenomena - drug effects
Cloning
Fibroblasts
Growth factors
Humans
Medical equipment
Methods
Physiological aspects
Polyesters - chemistry
Polyesters - pharmacology
Polymers
Stem cells
Stem Cells - chemistry
Stem Cells - cytology
Studies
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds - chemistry
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Summary:Bone tissue engineering is an emerging field, representing one of the most exciting challenges for scientists and clinicians. The possibility of combining mesenchymal stem cells and scaffolds to create engineered tissues has brought attention to a large variety of biomaterials in combination with osteoprogenitor cells able to promote and regenerate bone tissue. Human adipose tissue is officially recognized as an easily accessible source of mesenchymal stem cells (AMSCs), a significant factor for use in tissue regenerative medicine. In this study, we analyze the behavior of a clonal finite cell line derived from human adipose tissue seeded on poly(ε-caprolactone) (PCL) film, prepared by solvent casting. PCL polymer is chosen for its good biocompatibility, biodegradability, and mechanical properties. We observe that AMSCs are able to adhere to the biomaterial and remain viable for the entire experimental period. Moreover, we show that the proliferation process and osteogenic activity of AMSCs are maintained on the biofilm, demonstrating that the selected biomaterial ensures cell colonization and the development of an extracellular mineralized matrix. The results of this study highlight that AMSCs and PCL film can be used as a suitable model to support regeneration of new bone for future tissue engineering strategies.
ISSN:2314-6133
2314-6141
DOI:10.1155/2015/323571