In situ detection of TGF betas, TGF beta receptor II mRNA and telomerase activity in rat cholangiocarcinogenesis

Initial report on the in situ examination of the mRNA expression of transforming growth factor betas (TGFbetas), TGFbeta type II receptor (TbetaRII) and telomerase activity in the experimental rat liver tissue during cholangiocarcinogenesis. Rat liver cholangiocarcinogenesis was induced by 3'-m...

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Published in:World journal of gastroenterology : WJG 2003-03, Vol.9 (3), p.590-594
Main Authors: Lu, Jian-Ping, Mao, Jian-Qun, Li, Ming-Sheng, Lu, Shi-Lun, Hu, Xi-Qi, Zhu, Shi-Neng, Nomura, Shintaro
Format: Article
Language:English
Subjects:
Animals
Azo Compounds
Basic Research
Benzene Derivatives
Cholangiocarcinoma - chemically induced
Cholangiocarcinoma - metabolism
Male
Protein-Serine-Threonine Kinases
Rats
Rats, Wistar
Receptor, Transforming Growth Factor-beta Type II
Receptors, Transforming Growth Factor beta - genetics
RNA, Messenger - metabolism
Telomerase - metabolism
Transforming Growth Factor beta - genetics
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Summary:Initial report on the in situ examination of the mRNA expression of transforming growth factor betas (TGFbetas), TGFbeta type II receptor (TbetaRII) and telomerase activity in the experimental rat liver tissue during cholangiocarcinogenesis. Rat liver cholangiocarcinogenesis was induced by 3'-methyl 4-dimethylazobenzene (3'Me-DAB). In situ hybridization was used to examine the TGFbetas) and TGFbeta type II receptor (TbetaRII) mRNA, in situ TRAP was used to check the telomerase activity in the tissue samples. There was no TGFbetas, TbetaRII mRNA expression or telomerase activity in the control rat cholangiocytes. The expression of TGFbeta1, TbetaRII was increased in regenerative, hyperplastic, dysplastic cholangiocytes and cholangiocarcinoma (CC) cells. The expression of TGFbeta2 mRNA was observed in only a part of hyperplastic, dysplastic cholangiocytes. TGFbeta3 expression was very weak, only in hyperplastic lesion. There was positive telomerase activity in the regenerative, hyperplastic, dysplastic cholangiocytes, and CC cells. Stroma fibroblasts of these lesions also showed positive TGFbetas, TbetaRII mRNA expression and telomerase activity. There were TGFbetas, TbetaRII expression and telomerase activity in hyperplastic, dysplastic cholangiocytes, cholangiocarcinoma cells as well as in stroma fibroblasts during cholangiocarcinogenesis. Their expression or activity is important in cholangiocarcinogenesis andstroma formation.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v9.i3.590