Role of nucleostemin in growth regulation of gastric cancer,liver cancer and other malignancies

AIM:To examine the role of nucleostemin in the growth regulation of gastric cancer,liver cancer and other cancers.METHODS:RT-PCR was used to clone the fragment of nucleostemin cDNA from HEK 293 cells.Eighteen kinds of malignant tumor tissues including gastric adenocarcinoma and liver cancer tissues,...

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Published in:World journal of gastroenterology : WJG 2004-05, Vol.10 (9), p.1246-1249
Main Authors: Liu, Si-Jin, Cai, Zi-Wei, Liu, Ya-Jun, Dong, Mei-Yu, Sun, Li-Qiu, Hu, Guo-Fa, Wei, Ying-Yun, Lao, Wei-De
Format: Article
Language:English
Subjects:
Animals
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cell Line
Cloning, Molecular
Female
Gastric Cancer
Gene Expression Regulation
GTP-Binding Proteins
Humans
In Situ Hybridization
Kidney - cytology
Kidney - metabolism
Liver Neoplasms - genetics
Liver Neoplasms - metabolism
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Tissue Distribution
作用机制
细胞核蛋白
肿瘤生物学
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Summary:AIM:To examine the role of nucleostemin in the growth regulation of gastric cancer,liver cancer and other cancers.METHODS:RT-PCR was used to clone the fragment of nucleostemin cDNA from HEK 293 cells.Eighteen kinds of malignant tumor tissues including gastric adenocarcinoma and liver cancer tissues,3 kinds of benign tumor tissues,3 kinds of benign hyperplastic tissues and normal tissues were employed to examine nucleostemin gene expression by RT-PCR,Slot blot,Northern blot and in situ hybridization.RESULTS:We successfully cloned a 570 bp fragment of nucleostemin-cDNA from HEK-293 cells.All edtected malignant tumor tissues,benign tumor tissues,and benign hyperplastic tissues had hign levels of nucleostemin expression.Nucleostemin was also expressed in human placenta tissue at a high levle.In terminally differentiated normal human adult kidney and mammary gland tissues,no nucleostemin expression could be detected.CONCLUSION:Nucleostemin can help regulate the proliferation of both cancer cells and stem cells.It might play an important role in the growth regulation of gastric cancer,liver cancer and other cancers.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v10.i9.1246