Persistence of high CD40 and CD40L expression after restorative proctocolectomy for ulcerative colitis

AIM： To focus on the role of CD40 and CD40L in their pathogenesis. METHODS： We analyzed by immunohistochemistry the CD40 and CD40L expression in the pouch mucosa of 28 patients who had undergone RPC for UC, in the terminal ileum of 6 patients with UC and 11 healthy subjects. We also examined by flow...

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Published in:World journal of gastroenterology : WJG 2005-09, Vol.11 (34), p.5303-5308
Main Authors: Polese, Lino, Angriman, Imerio, Giuseppe, De Franchis, Cecchetto, Attilio, Sturniolo, Giacomo-C, Renata, D'Inca, Scarpa, Marco, Ruffolo, Cesare, Norberto, Lorenzo, Frego, Mauro, D'Amico, Davide-F
Format: Article
Language:English
Subjects:
Adult
CD40
CD40 Antigens - metabolism
CD40 Ligand - metabolism
CD40L
Clinical Research
Colitis, Ulcerative - metabolism
Colitis, Ulcerative - surgery
Female
Humans
Immunohistochemistry
Male
Middle Aged
Postoperative Complications - metabolism
Pouchitis - metabolism
Proctocolectomy, Restorative
基因表达
大肠炎
滋补剂
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Summary:AIM： To focus on the role of CD40 and CD40L in their pathogenesis. METHODS： We analyzed by immunohistochemistry the CD40 and CD40L expression in the pouch mucosa of 28 patients who had undergone RPC for UC, in the terminal ileum of 6 patients with UC and 11 healthy subjects. We also examined by flow cytometry the expression of CEH0 by B lymphoo/tes and monocytes in the peripheral blood of 20 pouch patients, 15 UC patients and 11 healthy controls. RESULTS： Ileal pouch mucosa leukocytes presented a significantly higher expression of CD40 and CD40L as compared to controls. This alteration correlated with pouchitis, but was also present in the healthy pouch and in the terminal ileum of UC patients. CD40 expression of peripheral B lymphocytes was significantly higher in patients with UC and pouch, respect to controls. Increased CD40 levels in blood B cells of pouch patients correlated with the presence of spondyloarthropathy, but not with pouchitis, or inflammatory indices. CONCLUSION： High CD40 expression in the ileal pouch mucosa could be implied in the pathogenesis of pouchitis following proctocolectomy for UC, whereas its increased levels on peripheral blood B lymphocytes are associated with the presence of extraintestinal manifestations.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v11.i34.5303