Protective effect of histamine microinjected into the cerebellar fastigial nucleus on stress-induced gastric mucosal damage in rats

• Published in: American journal of translational research 2015-01, Vol.7 (9), p.1648-1659
• Main Authors: Qiao, Xiao, Tang, Xiaolong, Zhang, Jianfu, Chen, Ke, Zhang, Yanming, Wang, Changcheng, Fei, Sujuan, Zhu, Jinzhou, Zhu, Shengping, Liu, Zhangbo, Li, Tingting, Lv, Shengxiang, Liang, Yong
• Format: Article
• Language: English
• Subjects: Original
• ISSN: 1943-8141; 1943-8141

We investigated the effffects and the possible mechanism of microinjection of histamine into cerebellar fastigial nucleus (FN) on stress-induced gastric mucosal damage (SGMD) in rats. The effect of microinjection of histamine into FN on SGMD was observed. The model of SGMD was established by restraint and water (21 ± 1°C)-immersion (RWI) for 3 h in rats. The gastric mucosal damage index indicated the severity of SGMD. Western blotting was performed to assess gastric mucosal cell apoptosis and proliferation. We observed that histamine microinjection into the FN markedly attenuated SGMD in a dose-dependent manner, and was prevented by pre-treatment with the ranitidine (a selective histamine H2 receptor antagonist) into the FN. The effect of histamine was abolished by pre-treatment with 3-MPA (a glutamic acid decarboxylase antagonist) into the FN. There was a decrease in the discharge frequency of greater splanchnic nerve, and an increase in gastric mucosal blood flow after histamine injection into the FN. Additionally, anti-apoptotic and anti-oxidative factors of gastric mucosa might be involved in this process. The exogenous histamine in FN participates in the regulation of SGMD, and our results may help to provide new ideas on the treatment of gastroenterological diseases.