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Rapid, simple and inexpensive production of custom 3D printed equipment for large-volume fluorescence microscopy
[Display omitted] The cost of 3D printing has reduced dramatically over the last few years and is now within reach of many scientific laboratories. This work presents an example of how 3D printing can be applied to the development of custom laboratory equipment that is specifically adapted for use w...
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| Published in: | International journal of pharmaceutics 2015-10, Vol.494 (2), p.651-656 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c537t-92b119be8ac49727b577f2a4f62fcb983af8641e45def2db4fdc757f565101db3 |
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| cites | cdi_FETCH-LOGICAL-c537t-92b119be8ac49727b577f2a4f62fcb983af8641e45def2db4fdc757f565101db3 |
| container_end_page | 656 |
| container_issue | 2 |
| container_start_page | 651 |
| container_title | International journal of pharmaceutics |
| container_volume | 494 |
| creator | Tyson, Adam L. Hilton, Stephen T. Andreae, Laura C. |
| description | [Display omitted]
The cost of 3D printing has reduced dramatically over the last few years and is now within reach of many scientific laboratories. This work presents an example of how 3D printing can be applied to the development of custom laboratory equipment that is specifically adapted for use with the novel brain tissue clearing technique, CLARITY. A simple, freely available online software tool was used, along with consumer-grade equipment, to produce a brain slicing chamber and a combined antibody staining and imaging chamber. Using standard 3D printers we were able to produce research-grade parts in an iterative manner at a fraction of the cost of commercial equipment. 3D printing provides a reproducible, flexible, simple and cost-effective method for researchers to produce the equipment needed to quickly adopt new methods. |
| doi_str_mv | 10.1016/j.ijpharm.2015.03.042 |
| format | article |
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The cost of 3D printing has reduced dramatically over the last few years and is now within reach of many scientific laboratories. This work presents an example of how 3D printing can be applied to the development of custom laboratory equipment that is specifically adapted for use with the novel brain tissue clearing technique, CLARITY. A simple, freely available online software tool was used, along with consumer-grade equipment, to produce a brain slicing chamber and a combined antibody staining and imaging chamber. Using standard 3D printers we were able to produce research-grade parts in an iterative manner at a fraction of the cost of commercial equipment. 3D printing provides a reproducible, flexible, simple and cost-effective method for researchers to produce the equipment needed to quickly adopt new methods.</description><identifier>ISSN: 0378-5173</identifier><identifier>EISSN: 1873-3476</identifier><identifier>DOI: 10.1016/j.ijpharm.2015.03.042</identifier><identifier>PMID: 25797056</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>3D printing ; Additive manufacturing ; Animals ; Brain - pathology ; CLARITY ; Mice ; Mice, Inbred C57BL ; Microscopy, Fluorescence - instrumentation ; Optical clearing ; Printing, Three-Dimensional - instrumentation ; Software</subject><ispartof>International journal of pharmaceutics, 2015-10, Vol.494 (2), p.651-656</ispartof><rights>2015 The Authors</rights><rights>Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.</rights><rights>2015 The Authors 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c537t-92b119be8ac49727b577f2a4f62fcb983af8641e45def2db4fdc757f565101db3</citedby><cites>FETCH-LOGICAL-c537t-92b119be8ac49727b577f2a4f62fcb983af8641e45def2db4fdc757f565101db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25797056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tyson, Adam L.</creatorcontrib><creatorcontrib>Hilton, Stephen T.</creatorcontrib><creatorcontrib>Andreae, Laura C.</creatorcontrib><title>Rapid, simple and inexpensive production of custom 3D printed equipment for large-volume fluorescence microscopy</title><title>International journal of pharmaceutics</title><addtitle>Int J Pharm</addtitle><description>[Display omitted]
The cost of 3D printing has reduced dramatically over the last few years and is now within reach of many scientific laboratories. This work presents an example of how 3D printing can be applied to the development of custom laboratory equipment that is specifically adapted for use with the novel brain tissue clearing technique, CLARITY. A simple, freely available online software tool was used, along with consumer-grade equipment, to produce a brain slicing chamber and a combined antibody staining and imaging chamber. Using standard 3D printers we were able to produce research-grade parts in an iterative manner at a fraction of the cost of commercial equipment. 3D printing provides a reproducible, flexible, simple and cost-effective method for researchers to produce the equipment needed to quickly adopt new methods.</description><subject>3D printing</subject><subject>Additive manufacturing</subject><subject>Animals</subject><subject>Brain - pathology</subject><subject>CLARITY</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microscopy, Fluorescence - instrumentation</subject><subject>Optical clearing</subject><subject>Printing, Three-Dimensional - instrumentation</subject><subject>Software</subject><issn>0378-5173</issn><issn>1873-3476</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqFUU1v1DAQtRCIbgs_AeQjBxL8EdvJBVQVSpEqVargbDn2uPUqiVM7WdF_j1e7VHDqaaSZN2_evIfQO0pqSqj8tK3Ddr43aawZoaImvCYNe4E2tFW84o2SL9GGcNVWgip-gk5z3hJCJKP8NTphQnWKCLlB862Zg_uIcxjnAbCZHA4T_J5hymEHeE7RrXYJccLRY7vmJY6Yfy39MC3gMDysYR5hWrCPCQ8m3UG1i8M6AvbDGhNkC5MFPAabYrZxfnyDXnkzZHh7rGfo1-W3nxdX1fXN9x8X59eVFVwtVcd6SrseWmObTjHVC6U8M42XzNu-a7nxrWwoNMKBZ65vvLNKKC-kKPa4np-hzwfeee1HcEXGksygi_DRpEcdTdD_T6Zwr-_iTjeSSaFYIfhwJEjxYYW86DGUb4bBTBDXrKkqXjZSiLZAxQG6fzIn8E9nKNH7tPRWH9PS-7Q04bqkVfbe_6vxaetvPAXw5QCA4tQuQNLZhr2hLiSwi3YxPHPiDxVprPA</recordid><startdate>20151030</startdate><enddate>20151030</enddate><creator>Tyson, Adam L.</creator><creator>Hilton, Stephen T.</creator><creator>Andreae, Laura C.</creator><general>Elsevier B.V</general><general>Elsevier/North-Holland Biomedical Press</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20151030</creationdate><title>Rapid, simple and inexpensive production of custom 3D printed equipment for large-volume fluorescence microscopy</title><author>Tyson, Adam L. ; Hilton, Stephen T. ; Andreae, Laura C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c537t-92b119be8ac49727b577f2a4f62fcb983af8641e45def2db4fdc757f565101db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>3D printing</topic><topic>Additive manufacturing</topic><topic>Animals</topic><topic>Brain - pathology</topic><topic>CLARITY</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microscopy, Fluorescence - instrumentation</topic><topic>Optical clearing</topic><topic>Printing, Three-Dimensional - instrumentation</topic><topic>Software</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tyson, Adam L.</creatorcontrib><creatorcontrib>Hilton, Stephen T.</creatorcontrib><creatorcontrib>Andreae, Laura C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of pharmaceutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tyson, Adam L.</au><au>Hilton, Stephen T.</au><au>Andreae, Laura C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid, simple and inexpensive production of custom 3D printed equipment for large-volume fluorescence microscopy</atitle><jtitle>International journal of pharmaceutics</jtitle><addtitle>Int J Pharm</addtitle><date>2015-10-30</date><risdate>2015</risdate><volume>494</volume><issue>2</issue><spage>651</spage><epage>656</epage><pages>651-656</pages><issn>0378-5173</issn><eissn>1873-3476</eissn><abstract>[Display omitted]
The cost of 3D printing has reduced dramatically over the last few years and is now within reach of many scientific laboratories. This work presents an example of how 3D printing can be applied to the development of custom laboratory equipment that is specifically adapted for use with the novel brain tissue clearing technique, CLARITY. A simple, freely available online software tool was used, along with consumer-grade equipment, to produce a brain slicing chamber and a combined antibody staining and imaging chamber. Using standard 3D printers we were able to produce research-grade parts in an iterative manner at a fraction of the cost of commercial equipment. 3D printing provides a reproducible, flexible, simple and cost-effective method for researchers to produce the equipment needed to quickly adopt new methods.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25797056</pmid><doi>10.1016/j.ijpharm.2015.03.042</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-5173 |
| ispartof | International journal of pharmaceutics, 2015-10, Vol.494 (2), p.651-656 |
| issn | 0378-5173 1873-3476 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4626572 |
| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | 3D printing Additive manufacturing Animals Brain - pathology CLARITY Mice Mice, Inbred C57BL Microscopy, Fluorescence - instrumentation Optical clearing Printing, Three-Dimensional - instrumentation Software |
| title | Rapid, simple and inexpensive production of custom 3D printed equipment for large-volume fluorescence microscopy |
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