RETRACTED ARTICLE: In vitro derivation of mammalian germ cells from stem cells and their potential therapeutic application

• Published in: Cellular and molecular life sciences : CMLS 2015-12, Vol.72 (23), p.4545-4560
• Main Authors: Saito, Shigeo, Lin, Ying-Chu, Murayama, Yoshinobu, Nakamura, Yukio, Eckner, Richard, Niemann, Heiner, Yokoyama, Kazunari K.
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell Biology; Life Sciences; Review
• ISSN: 1420-682X; 1420-9071
• DOI: 10.1007/s00018-015-2020-1

Pluripotent stem cells (PSCs) are a unique type of cells because they exhibit the characteristics of self-renewal and pluripotency. PSCs may be induced to differentiate into any cell type, even male and female germ cells, suggesting their potential as novel cell-based therapeutic treatment for infertility problems. Spermatogenesis is an intricate biological process that starts from self-renewal of spermatogonial stem cells (SSCs) and leads to differentiated haploid spermatozoa. Errors at any stage in spermatogenesis may result in male infertility. During the past decade, much progress has been made in the derivation of male germ cells from various types of progenitor stem cells. Currently, there are two main approaches for the derivation of functional germ cells from PSCs, either the induction of in vitro differentiation to produce haploid cell products, or combination of in vitro differentiation and in vivo transplantation. The production of mature and fertile spermatozoa from stem cells might provide an unlimited source of autologous gametes for treatment of male infertility. Here, we discuss the current state of the art regarding the differentiation potential of SSCs, embryonic stem cells, and induced pluripotent stem cells to produce functional male germ cells. We also discuss the possible use of livestock-derived PSCs as a novel option for animal reproduction and infertility treatment.