Quantification of the concentration gradient of biomarkers between ovarian carcinoma interstitial fluid and blood

Tumor interstitial fluid (TIF) rather than plasma should be used in cancer biomarker discovery because of the anticipated higher concentration of locally produced proteins in the tumor microenvironment. Nevertheless, the actual TIF-to-plasma gradient of tumor specific proteins has not been quantifie...

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Bibliographic Details
Published in:BBA clinical 2014-12, Vol.2, p.18-23
Main Authors: Haslene-Hox, Hanne, Madani, Amina, Berg, Kaja C.G., Woie, Kathrine, Salvesen, Helga B., Wiig, Helge, Tenstad, Olav
Format: Article
Language:English
Subjects:
Biomarker
CA-125
Ovarian carcinoma
Regular
Tumor interstitial fluid
Tumor microenvironment
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Summary:Tumor interstitial fluid (TIF) rather than plasma should be used in cancer biomarker discovery because of the anticipated higher concentration of locally produced proteins in the tumor microenvironment. Nevertheless, the actual TIF-to-plasma gradient of tumor specific proteins has not been quantified. We present the proof-of-concept for the quantification of the postulated gradient between TIF and plasma. TIF was collected by centrifugation from serous (n=19), endometrioid (n=9) and clear cell (n=3) ovarian carcinomas with early (n=15) and late stage (n=16) disease in grades 1 (n=2), 2 (n=8) and 3 (n=17), and ELISA was used for the determination of CA-125, osteopontin and VEGF-A. All three markers were significantly up-regulated in TIF compared with plasma (p
ISSN:2214-6474
2214-6474
DOI:10.1016/j.bbacli.2014.08.002