Use of microsatellite and SNP markers for biotype characterization in Hessian fly

Exploration of the biotype structure of Hessian fly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae), would improve our knowledge regarding variation in virulence phenotypes and difference in genetic background. Microsatellites (simple sequence repeats) and single-nucleotide polymorphisms (SNPs)...

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Bibliographic Details
Published in:Journal of insect science (Tucson, Ariz.) Ariz.), 2015, Vol.15 (1), p.158
Main Authors: Schemerhorn, Brandon J, Crane, Yan Ma, Cambron, Sue E, Crane, Charles F, Shukle, Richard H
Format: Article
Language:English
Subjects:
Animals
Diptera - classification
Diptera - genetics
DNA - genetics
Genes, Insect
Genes, Plant
Genetic Markers
Microsatellite Repeats
Phenotype
Polymorphism, Single Nucleotide
Triticum - genetics
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Summary:Exploration of the biotype structure of Hessian fly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae), would improve our knowledge regarding variation in virulence phenotypes and difference in genetic background. Microsatellites (simple sequence repeats) and single-nucleotide polymorphisms (SNPs) are highly variable genetic markers that are widely used in population genetic studies. This study developed and tested a panel of 18 microsatellite and 22 SNP markers to investigate the genetic structure of nine Hessian fly biotypes: B, C, D, E, GP, L, O, vH9, and vH13. The simple sequence repeats were more polymorphic than the SNP markers, and their neighbor-joining trees differed in consequence. Microsatellites suggested a simple geographic association of related biotypes that did not progressively gain virulence with increasing genetic distance from a founder type. Use of the k-means clustering algorithm in the STRUCTURE program shows that the nine biotypes comprise six to eight populations that are related to geography or history within laboratory cultures.
ISSN:1536-2442
1536-2442
DOI:10.1093/jisesa/iev138