Genotyping coronaviruses associated with feline infectious peritonitis

Feline coronavirus (FCoV) infections are endemic among cats worldwide. The majority of infections are asymptomatic or result in only mild enteric disease. However, approximately 5 % of cases develop feline infectious peritonitis (FIP), a systemic disease that is a frequent cause of death in young ca...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general virology 2015-06, Vol.96 (Pt 6), p.1358-1368
Main Authors: Lewis, Catherine S, Porter, Emily, Matthews, David, Kipar, Anja, Tasker, Séverine, Helps, Christopher R, Siddell, Stuart G
Format: Article
Language:English
Subjects:
Animal
Animals
Cats
Cluster Analysis
Coronavirus, Feline - classification
Coronavirus, Feline - genetics
Coronavirus, Feline - isolation & purification
Feline Infectious Peritonitis - virology
Gene Order
Genes, Viral
Genetic Variation
Genome, Viral
Genotype
Molecular Sequence Data
Phylogeny
RNA, Viral - genetics
Sequence Analysis, DNA
Sequence Homology
United Kingdom
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Feline coronavirus (FCoV) infections are endemic among cats worldwide. The majority of infections are asymptomatic or result in only mild enteric disease. However, approximately 5 % of cases develop feline infectious peritonitis (FIP), a systemic disease that is a frequent cause of death in young cats. In this study, we report the complete coding genome sequences of six FCoVs: three from faecal samples from healthy cats and three from tissue lesion samples from cats with confirmed FIP. The six samples were obtained over a period of 8 weeks at a single-site cat rescue and rehoming centre in the UK. We found amino acid differences located at 44 positions across an alignment of the six virus translatomes and, at 21 of these positions, the differences fully or partially discriminated between the genomes derived from the faecal samples and the genomes derived from the tissue lesion samples. In this study, two amino acid differences fully discriminated the two classes of genomes: these were both located in the S2 domain of the virus surface glycoprotein gene. We also identified deletions in the 3c protein ORF of genomes from two of the FIP samples. Our results support previous studies that implicate S protein mutations in the pathogenesis of FIP.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.000084