Loading…
Influence of the protocol of fibroin extraction on the antibiotic activities of the constructed composites
The effect of the solvents for silk fibroin (SF) extraction on its antimicrobial activity was studied. Extraction protocols were performed using LiBr (SF L ) and Ajisawa’s reagent (CaCl 2 :ethanol:H 2 O) (SF C ). The morphological and structural characteristics of the extracted SF and their composit...
Saved in:
Published in: | Progress in biomaterials 2015, Vol.4 (2-4), p.77-88 |
---|---|
Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | The effect of the solvents for silk fibroin (SF) extraction on its antimicrobial activity was studied. Extraction protocols were performed using LiBr (SF
L
) and Ajisawa’s reagent (CaCl
2
:ethanol:H
2
O) (SF
C
). The morphological and structural characteristics of the extracted SF and their composites were assessed. Corresponding bactericidal activities against
Staphylococcus aureus
(ATCC 25923),
Escherichia coli
(ATCC 25922) and
Pseudomonas aeroginosa
(ATCC 27853) were performed. The resulting solutions were either casted into films or individually incorporated into composites of silver nanoparticles (NS) embedded into chitosan fragments (Cs) through γ-irradiation. Films of SF, obtained by using the two solvents, as well as the final prepared composites of SF, NS and Cs were analyzed using XRD, FTIR, SEM, TEM and zeta potential at several pH values. The band gap values were calculated. The results proved that, although SF
C
consumed shorter gelation time, yet SF
L
exerted higher antibiotic activity against the tested microorganisms. Moreover, the final composites had the ability to significantly reduce the growth of these medically relevant bacteria and are, therefore, recommended as a novel natural antibacterial biomaterial for several biomedical applications. |
---|---|
ISSN: | 2194-0509 2194-0517 |
DOI: | 10.1007/s40204-015-0039-x |