Human Cytomegalovirus pUL93 Is Required for Viral Genome Cleavage and Packaging

Human cytomegalovirus (HCMV) pUL93 is essential for virus growth, but its precise function in the virus life cycle is unknown. Here, we characterize a UL93 stop mutant virus (UL93st-TB40/E-BAC) to demonstrate that the absence of this protein does not restrict viral gene expression; however, cleavage...

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Bibliographic Details
Published in:Journal of virology 2015-12, Vol.89 (23), p.12221-12225
Main Authors: DeRussy, Bernadette M, Tandon, Ritesh
Format: Article
Language:English
Subjects:
Capsid Proteins - genetics
Capsid Proteins - metabolism
Codon, Nonsense - genetics
Cytomegalovirus - genetics
Cytomegalovirus - physiology
Gene Expression Regulation, Viral - genetics
Genetic Engineering - methods
Human cytomegalovirus
Humans
Microscopy, Electron, Transmission
Structure and Assembly
Viral Proteins - genetics
Viral Proteins - metabolism
Virus Assembly - genetics
Virus Assembly - physiology
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Summary:Human cytomegalovirus (HCMV) pUL93 is essential for virus growth, but its precise function in the virus life cycle is unknown. Here, we characterize a UL93 stop mutant virus (UL93st-TB40/E-BAC) to demonstrate that the absence of this protein does not restrict viral gene expression; however, cleavage of viral DNA into unit-length genomes as well as genome packaging is abolished. Thus, pUL93 is required for viral genome cleavage and packaging.
ISSN:0022-538X
1098-5514
DOI:10.1128/JVI.02382-15