Sequence Variation in HSP40 Gene among 16 Toxoplasma gondii Isolates from Different Hosts and Geographical Locations

Toxoplasma gondii with worldwide distribution has received substantial medical and scientific attentions as it causes serious clinical and veterinary problems especially for pregnant women and immunocompromised patients. Heat shock protein 40 (HSP40) plays a variety of essential roles in the pathoge...

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Bibliographic Details
Published in:BioMed research international 2015-01, Vol.2015 (2015), p.1-6
Main Authors: Chen, Jia, Zhou, Dong-Hui, Lu, Jing, Li, Zhong-Yuan, Zhu, Xing-Quan
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biomedical research
Cats
Deer - parasitology
Distribution
Genetic aspects
Genetic Variation - genetics
Genotype
Goats - parasitology
Heat shock proteins
HSP40 Heat-Shock Proteins - genetics
Humans
Laboratories
Molecular Sequence Data
Phylogenetics
Phylogeny
Physiological aspects
Protein folding
Protozoa
Protozoan Proteins - genetics
Puma - parasitology
Rodents
Science
Sequence Alignment
Sheep - parasitology
Signal transduction
Swine - parasitology
Thermal cycling
Toxoplasma
Toxoplasma - genetics
Toxoplasma - isolation & purification
Toxoplasma - parasitology
Toxoplasma gondii
Veterinary medicine
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Summary:Toxoplasma gondii with worldwide distribution has received substantial medical and scientific attentions as it causes serious clinical and veterinary problems especially for pregnant women and immunocompromised patients. Heat shock protein 40 (HSP40) plays a variety of essential roles in the pathogenesis of this protozoan parasite. In order to detail the genetic diversity of HSP40 gene, 16 T. gondii strains from different hosts and geographical locations were used in this study. Our results showed that HSP40 sequence of the examined strains was between 6621 bp and 6644 bp in length, and their A+T content was from 48.54% to 48.80%. Furthermore, sequence analysis presented 195 nucleotide mutation positions (0.12%–1.14%) including 29 positions in CDS (0.02%–0.12%) compared with T. gondii ME49 strain (ToxoDB: TGME49_265310). Phylogenetic assay revealed that T. gondii strains representing three classical genotypes (Types I, II, and III) were completely separated into different clusters by maximum parsimony (MP) method, but Type II and ToxoDB#9 strains were grouped into the same cluster. These results suggested that HSP40 gene is not a suitable marker for T. gondii population genetic research, though three classical genotypes of T. gondii could be differentiated by restriction enzymes MscI and EarI existing in amplicon C.
ISSN:2314-6133
2314-6141
DOI:10.1155/2015/209792