Peritoneal VEGF-A expression is regulated by TGF-β1 through an ID1 pathway in women with endometriosis

• Published in: Scientific reports 2015-11, Vol.5 (1), p.16859, Article 16859
• Main Authors: Young, Vicky J., Ahmad, Syed F., Brown, Jeremy K., Duncan, W. Colin, Horne, Andrew W.
• Format: Article
• Language: English
• Subjects: 13/51; 38; 38/109; 45/77; 692/699/249/2510; 692/699/2732/2730; 96/106; 96/63; Ascitic Fluid - metabolism; Cells, Cultured; Endometriosis - genetics; Endometriosis - metabolism; Female; Gene Expression Regulation - drug effects; Gene Knockdown Techniques; Humanities and Social Sciences; Humans; Inhibitor of Differentiation Protein 1 - genetics; Inhibitor of Differentiation Protein 1 - metabolism; multidisciplinary; Peritoneum - cytology; Peritoneum - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Small Interfering - genetics; Science; Signal Transduction; Transforming Growth Factor beta1 - metabolism; Transforming Growth Factor beta1 - pharmacology; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep16859

VEGF-A, an angiogenic factor, is increased in the peritoneal fluid of women with endometriosis. The cytokine TGF-β1 is thought to play a role in the establishment of endometriosis lesions. Inhibitor of DNA binding (ID) proteins are transcriptional targets of TGF-β1 and ID1 has been implicated in VEGF-A regulation during tumor angiogenesis. Herein, we determined whether peritoneal expression of VEGF-A is regulated by TGF-β1 through the ID1 pathway in women with endometriosis. VEGF-A was measured in peritoneal fluid by ELISA (n = 16). VEGF-A and ID1 expression was examined in peritoneal biopsies (n = 13) and primary peritoneal and immortalized mesothelial cells (MeT5A) by immunohistochemistry, qRT-PCR and ELISA. VEGF-A was increased in peritoneal fluid from women with endometriosis and levels correlated with TGF-β1 concentrations ( P