Oligomerization of Vibrio cholerae Hemolysin Induces CXCR3 Upregulation and Activation of B-1a Cell

• Published in: Cellular & molecular immunology 2008-06, Vol.5 (3), p.231-234
• Main Authors: Mukherjee, Gayatri, Banerjee, Kalyan K, Biswas, Tapas
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; B-1a细胞; B-Lymphocytes - immunology; B7-2 Antigen - immunology; B7-2 Antigen - metabolism; Bacterial Proteins - immunology; Bacterial Proteins - metabolism; Biomedical and Life Sciences; Biomedicine; Brief Report; CD25 antigen; CD86; CD86 antigen; Cell activation; Cell proliferation; Cells, Cultured; Chemokines; CXCL4; CXCR3; CXCR3 protein; Hemolysin Proteins - immunology; Hemolysin Proteins - metabolism; Immunology; Lymphocyte Activation; Medical Microbiology; Mice; Microbiology; Oligomerization; Oligomers; Platelet Factor 4 - immunology; Platelet Factor 4 - metabolism; Receptors, CXCR3 - immunology; Receptors, CXCR3 - metabolism; Up-Regulation; Vaccine; Vibrio cholerae; Vibrio cholerae - immunology; Vibrio cholerae - metabolism; 溶血素
• ISSN: 1672-7681; 2042-0226
• DOI: 10.1038/cmi.2008.29

The hemolysin oligomer promotes the proliferation of B-1a cells and the expression of CD25, which is indicative of cell activation, on B-1a cells. The upregulation of CD86 induced by the oligomer showed its selective bias for the B7-2 member of B7 family while the monomer failed to induce these effects. The oligomer induced the expression of CXCR3, associated with B cell activation, while the monomer induced the expression of CXCL4, a powerful angiostatic chemokine. In conclusion, we found that B-1a cells responded to the apoptogenic monomer by expressing CXCL4, whereas oligomerization of the immunogen induced CXCR3 to shift the response towards activation.