Targeting YAP-dependent MDSC infiltration impairs tumor progression

• Published in: Journal for immunotherapy of cancer 2015-11, Vol.3 (Suppl 2), p.P421-P421, Article P421
• Main Authors: Wang, Guocan, Lu, Xin, Dey, Prasenjit, Deng, Pingna, Wu, Chia Chin, Jiang, Shan, Fang, Zhuangna, Zhao, Kun, Konaparthi, Ramakrishna, Hua, Sujun, Zhang, Jianhua, Li Ning Tapia, Elsa M, Kapoor, Avnish, Patel, Neelay, Guo, Zhenglin, Ramamoorthy, Vandhana, Tieu, Trang, Heffernan, Tim, Zhao, Di, Shang, Xiaoying, Khadka, Sunada, Hou, Pingping, Hu, Baoli, Chang, Qing, Troncoso, Patricia, Logothetis, Christopher, McArthur, Mark, Chin, Lynda, Wang, Y Alan, DePinho, Ronald
• Format: Article
• Language: English
• Subjects: Cellular signal transduction; Development and progression; Gene expression; Genetic aspects; Health aspects; Immunotherapy; Poster Presentation; Prostate cancer; Tumor proteins
• ISSN: 2051-1426; 2051-1426
• DOI: 10.1186/2051-1426-3-S2-P421

BackgroundMyeloid-derived suppressor cells (MDSCs) represent a phenotypically heterogeneous population of immature myeloid cells that play a tumor-promoting role by maintaining a state of immunological anergy and tolerance. Similar to other solid tumor types, Prostate cancer (PCa) is characterized by a rich tumor-stroma interaction network that forms the TME. MDSC abundance in the blood correlates with circulating Prostate Specific Antigen (PSA) levels in PCa patients. MDSCs have been identified recently as a TME constituent in an indolent PCa mouse model with conditional Pten deletion, which antagonized senescence during early tumorigenesis. However, the molecular signaling mechanisms and their cellular origins underlying the recruitment of MDSCs are not well understood and the extent to which MDSCs facilitate PCa progression has not been determined.ResultsThe signaling mechanisms between cancer cells and infiltrating immune cells of prostate cancer may illuminate novel therapeutic approaches. Here, utilizing a murine prostate adenocarcinoma model driven by loss of Pten and Smad4 (Pb-Cre; PtenL/L;Smad4 L/L), we identify polymorphonuclear myeloid-derived suppressor cells (MDSCs) as the major infiltrating immune cell type and depletion of MDSCs blocks progression. Employing a dual cancer cell and host cell reporter prostate cancer model system (Pb-Cre; PtenL/L;Smad4 L/L;Rosa26-mTmGL/+), transcriptomic profiling of epithelial and stromal constituents identified Cxcl5 as a cancer-secreted chemokine to attract Cxcr2-expressing MDSCs and, correspondingly, pharmacological inhibition of Cxcr2 impeded tumor progression. Integrated analyses identified hyperactivated Hippo-YAP signaling in driving Cxcl5 upregulation in cancer cells through YAP-TEAD complex and promoting MDSCs recruitment. Clinico-pathological studies reveal upregulation and activation of YAP1 in a subset of human prostate tumors, and the YAP1 signature is enriched in these primary prostate tumor samples with stronger expression of MDSC relevant genes. Together, YAP-driven MDSC recruitment via heterotypic Cxcl5-Cxcr2 signaling, which promotes prostate tumor progression, reveals effective anti-MDSC therapeutic interventions for advanced prostate cancer.SignificanceThis study employs a novel autochthonous prostate cancer model to demonstrate a critical role of MDSCs in tumor progression. In addition, we discover a cancer cell non-autonomous function of Hippo-YAP pathway in regulation of Cxcl5, a ligand