Changes of tumor microcirculation after transcatheter arterial chemoembolization： First pass perfusion MR imaging and Chinese ink casting in a rabbit model

• Published in: World journal of gastroenterology : WJG 2004-05, Vol.10 (10), p.1415-1420
• Main Authors: Zhao, Jun-Gong, Feng, Gan-Sheng, Kong, Xiang-Quan, Li, Xin, Li, Ming-Hua, Cheng, Ying-Sheng
• Format: Article
• Language: English
• Subjects: Animals; Carbon - metabolism; Carcinoma, Hepatocellular - blood supply; Carcinoma, Hepatocellular - drug therapy; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Chemoembolization, Therapeutic - methods; Contrast Media - therapeutic use; Diagnostic Imaging; Female; Fluorouracil - therapeutic use; Iodized Oil - therapeutic use; Liver - cytology; Liver - metabolism; Liver - pathology; Liver Cancer; Liver Neoplasms, Experimental - blood supply; Liver Neoplasms, Experimental - drug therapy; Liver Neoplasms, Experimental - metabolism; Liver Neoplasms, Experimental - pathology; Magnetic Resonance Imaging - methods; Male; Microcirculation; Microspheres; Neoplasm Transplantation; Neovascularization, Pathologic; Rabbits; Random Allocation; 动物模型; 动脉导管化疗栓塞; 核磁共振; 肿瘤微循环; 肿瘤治疗学
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v10.i10.1415

AIM: To observe the change of tumor microcirculation after transcatheter arterial chemoembolization (TACE) with bletilla microspheres by using first pass perfusion MR imaging (FP) and Chinese ink casting.METHODS: VX2 carcinoma cells were surgically implanted into the left and right lobes of liver of 30 New Zealand white rabbits, which were divided into 3 groups at random. Emulsion of lipiodol mixed with mitomydn C, and 5-FU bletilla microspheres were injected into the hepatic artery respectively, and saline was used as control agent. MR imaging was performed with turbo-flash sequence 14 d after tumor implantation and 7 d after interventional therapy. The steepest slopes (SS) of the signal intensity versus time curves were created for quantitative analysis, 7.5% Chinese ink gelatin solution was injected through ascending artery (17 cases) or portal vein(2 cases) for lesion microvessel area (MVA) measurement after the last MRI examination.The correlation between perfusion imaging and MVA was studied blindly.RESULTS: The SS values at the rim of tumor in lipiodol group (mean, 49% per second) and bletilla group (mean,35% per second) were significantly decreased (P0.05). In lipiodol group, the MVAs (24 974±11 836μm^2) in the center of the tumor were significantly smaller than those of the control group (35 510±15 675 μm^2) (P